1sg5
From Proteopedia
(New page: 200px<br /><applet load="1sg5" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sg5" /> '''Solution structure of Yaeo, a Rho-specific i...) |
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'''Solution structure of Yaeo, a Rho-specific inhibitor of transcription termination'''<br /> | '''Solution structure of Yaeo, a Rho-specific inhibitor of transcription termination'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1SG5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | + | 1SG5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SG5 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 13:48:40 2008'' |
Revision as of 11:48, 23 January 2008
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Solution structure of Yaeo, a Rho-specific inhibitor of transcription termination
Overview
Rho-dependent transcription termination is an essential process for the, regulation of bacterial gene expression. Thus far, only two Rho-specific, inhibitors of bacterial transcription termination have been described, the, psu protein from the satellite bacteriophage P4 and YaeO from Escherichia, coli. Here, we report the solution structure of YaeO, the first of a, Rho-specific inhibitor of transcription termination. YaeO is an acidic, protein composed of an N-terminal helix and a seven-stranded beta, sandwich. NMR chemical shift perturbation experiments revealed that YaeO, binds proximal to the primary nucleic acid binding site of Rho. Based on, the NMR titrations, a docked model of the YaeO-Rho complex was calculated., These results suggest that YaeO binds outside the Rho hexamer, acting as a, competitive inhibitor of RNA binding. In vitro gel shift assays confirmed, the inhibition of nucleic acid binding to Rho. Site-directed mutagenesis, showed that the negative character of YaeO is essential for its function, in vivo.
About this Structure
1SG5 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Solution Structure of YaeO, a Rho-specific Inhibitor of Transcription Termination., Gutierrez P, Kozlov G, Gabrielli L, Elias D, Osborne MJ, Gallouzi IE, Gehring K, J Biol Chem. 2007 Aug 10;282(32):23348-53. Epub 2007 Jun 11. PMID:17565995
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