2gmn
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(New page: 200px<br /><applet load="2gmn" size="350" color="white" frame="true" align="right" spinBox="true" caption="2gmn, resolution 1.40Å" /> '''Crystal structure of...)
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Revision as of 11:55, 23 January 2008
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Crystal structure of BJP-1, a subclass B3 metallo-beta-lactamase of Bradyrhizobium japonicum
Overview
The diffusion of metallo-beta-lactamases (MBLs) among clinically important, human pathogens represents a therapeutic issue of increasing importance., However, the origin of these resistance determinants is largely unknown, although an important number of proteins belonging to the MBL superfamily, have been identified in microbial genomes. In this work, we analyzed the, distribution and function of genes encoding MBL-like proteins in the class, Rhizobiales. Among 12 released complete genomes of members of the class, Rhizobiales, a total of 57 open reading frames (ORFs) were found to have, the MBL conserved motif and identity scores with MBLs ranging from 8 to, 40%. On the basis of the best identity scores with known MBLs, four ORFs, were cloned into Escherichia coli for heterologous expression. Among their, products, one (blr6230) encoded by the Bradyrhizobium japonicum USDA110, genome, named BJP-1, hydrolyzed beta-lactams when expressed in E. coli., BJP-1 enzyme is most closely related to the CAU-1 enzyme from Caulobacter, vibrioides (40% amino acid sequence identity), a member of subclass B3, MBLs. A kinetic analysis revealed that BJP-1 efficiently hydrolyzed most, beta-lactam substrates, except aztreonam, ticarcillin, and temocillin, with the highest catalytic efficiency measured with meropenem. Compared to, other MBLs, BJP-1 was less sensitive to inactivation by chelating agents.
About this Structure
2GMN is a Single protein structure of sequence from Bradyrhizobium japonicum with as ligand. Active as Beta-lactamase, with EC number 3.5.2.6 Full crystallographic information is available from OCA.
Reference
Postgenomic scan of metallo-beta-lactamase homologues in rhizobacteria: identification and characterization of BJP-1, a subclass B3 ortholog from Bradyrhizobium japonicum., Stoczko M, Frere JM, Rossolini GM, Docquier JD, Antimicrob Agents Chemother. 2006 Jun;50(6):1973-81. PMID:16723554
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