2ool
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(New page: 200px<br /><applet load="2ool" size="350" color="white" frame="true" align="right" spinBox="true" caption="2ool, resolution 2.20Å" /> '''Crystal structure of...)
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Revision as of 11:56, 23 January 2008
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Crystal structure of the chromophore-binding domain of an unusual bacteriophytochrome RpBphP3 from R. palustris
Overview
Bacteriophytochromes RpBphP2 and RpBphP3 from the photosynthetic bacterium, Rhodopseudomonas palustris work in tandem to modulate synthesis of the, light-harvesting complex LH4 in response to light. Although RpBphP2 and, RpBphP3 share the same domain structure with 52% sequence identity, they, demonstrate distinct photoconversion behaviors. RpBphP2 exhibits the, "classical" phytochrome behavior of reversible photoconversion between red, (Pr) and far-red (Pfr) light-absorbing states, whereas RpBphP3 exhibits, novel photoconversion between Pr and a near-red (Pnr) light-absorbing, states. We have determined the crystal structure at 2.2-A resolution of, the chromophore binding domains of RpBphP3, covalently bound with, chromophore biliverdin IXalpha. By combining structural and sequence, analyses with site-directed mutagenesis, we identify key residues that, directly modulate the photochemical properties of RpBphP3 and RpBphP2., Remarkably, we identify a region spanning residues 207-212 in RpBphP3, in, which a single mutation, L207Y, causes this unusual bacteriophytochrome to, revert to the classical phenotype that undergoes reversible, photoconversion between the Pr and Pfr states. The reverse mutation, Y193L, in the corresponding region in RpBphP2 significantly diminishes the, formation of the Pfr state. We propose that residues 207-212 and the, spatially adjacent conserved residues, Asp-216 and Tyr-272, interact with, the chromophore and form part of the interface between the chromophore, binding domains and the PHY domain that modulates photoconversion.
About this Structure
2OOL is a Single protein structure of sequence from Rhodopseudomonas palustris with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of the chromophore binding domain of an unusual bacteriophytochrome, RpBphP3, reveals residues that modulate photoconversion., Yang X, Stojkovic EA, Kuk J, Moffat K, Proc Natl Acad Sci U S A. 2007 Jul 24;104(30):12571-6. Epub 2007 Jul 17. PMID:17640891
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