1aa6
From Proteopedia
(New page: 200px<br /> <applet load="1aa6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1aa6, resolution 2.3Å" /> '''REDUCED FORM OF FORM...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1AA6 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with SF4, MGD and 4MO as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.2 1.2.1.2]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AA6 OCA]]. | + | 1AA6 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with SF4, MGD and 4MO as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Formate_dehydrogenase Formate dehydrogenase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.2 1.2.1.2]]. Structure known Active Sites: 4MO and FS4. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AA6 OCA]]. |
==Reference== | ==Reference== | ||
Crystal structure of formate dehydrogenase H: catalysis involving Mo, molybdopterin, selenocysteine, and an Fe4S4 cluster., Boyington JC, Gladyshev VN, Khangulov SV, Stadtman TC, Sun PD, Science. 1997 Feb 28;275(5304):1305-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9036855 9036855] | Crystal structure of formate dehydrogenase H: catalysis involving Mo, molybdopterin, selenocysteine, and an Fe4S4 cluster., Boyington JC, Gladyshev VN, Khangulov SV, Stadtman TC, Sun PD, Science. 1997 Feb 28;275(5304):1305-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9036855 9036855] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
| + | [[Category: Formate dehydrogenase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Boyington, J.C.]] | [[Category: Boyington, J.C.]] | ||
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[[Category: selenocysteine]] | [[Category: selenocysteine]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:37:59 2007'' |
Revision as of 09:33, 30 October 2007
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REDUCED FORM OF FORMATE DEHYDROGENASE H FROM E. COLI
Overview
Formate dehydrogenase H from Escherichia coli contains selenocysteine, (SeCys), molybdenum, two molybdopterin guanine dinucleotide (MGD), cofactors, and an Fe4S4 cluster at the active site and catalyzes the, two-electron oxidation of formate to carbon dioxide. The crystal, structures of the oxidized [Mo(VI), Fe4S4(ox)] form of formate, dehydrogenase H (with and without bound inhibitor) and the reduced, [Mo(IV), Fe4S4(red)] form have been determined, revealing a four-domain, alphabeta structure with the molybdenum directly coordinated to selenium, and both MGD cofactors. These structures suggest a reaction mechanism that, directly involves SeCys140 and His141 in proton abstraction and the, molybdenum, molybdopterin, Lys44, and the Fe4S4 cluster in electron, transfer.
About this Structure
1AA6 is a [Single protein] structure of sequence from [Escherichia coli] with SF4, MGD and 4MO as [ligands]. Active as [Formate dehydrogenase], with EC number [1.2.1.2]. Structure known Active Sites: 4MO and FS4. Full crystallographic information is available from [OCA].
Reference
Crystal structure of formate dehydrogenase H: catalysis involving Mo, molybdopterin, selenocysteine, and an Fe4S4 cluster., Boyington JC, Gladyshev VN, Khangulov SV, Stadtman TC, Sun PD, Science. 1997 Feb 28;275(5304):1305-8. PMID:9036855
Page seeded by OCA on Tue Oct 30 11:37:59 2007
Categories: Escherichia coli | Formate dehydrogenase | Single protein | Boyington, J.C. | Sun, P.D. | 4MO | MGD | SF4 | Anaerobic | Dehydrogenase | Fe4s4 | Formate | Iron sulfur cluster | Mgd | Molybdenum | Molybdopterin | Molybdopterin guanine dinucleotide | Mpt | Oxidoreductase | Secys | Selenium | Selenocysteine
