2iw0
From Proteopedia
(New page: 200px<br /> <applet load="2iw0" size="450" color="white" frame="true" align="right" spinBox="true" caption="2iw0, resolution 1.81Å" /> '''STRUCTURE OF THE CH...) |
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==About this Structure== | ==About this Structure== | ||
| - | 2IW0 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/ | + | 2IW0 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Glomerella_lindemuthiana Glomerella lindemuthiana]] with ZN, ACT, CL and PO4 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Chitin_deacetylase Chitin deacetylase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.41 3.5.1.41]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2IW0 OCA]]. |
==Reference== | ==Reference== | ||
Structure and mechanism of chitin deacetylase from the fungal pathogen Colletotrichum lindemuthianum., Blair DE, Hekmat O, Schuttelkopf AW, Shrestha B, Tokuyasu K, Withers SG, van Aalten DM, Biochemistry. 2006 Aug 8;45(31):9416-26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16878976 16878976] | Structure and mechanism of chitin deacetylase from the fungal pathogen Colletotrichum lindemuthianum., Blair DE, Hekmat O, Schuttelkopf AW, Shrestha B, Tokuyasu K, Withers SG, van Aalten DM, Biochemistry. 2006 Aug 8;45(31):9416-26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16878976 16878976] | ||
| - | [[Category: | + | [[Category: Chitin deacetylase]] |
| + | [[Category: Glomerella lindemuthiana]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Aalten, D.M.F.Van.]] | [[Category: Aalten, D.M.F.Van.]] | ||
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[[Category: hydrolase]] | [[Category: hydrolase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:38:13 2007'' |
Revision as of 09:33, 30 October 2007
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STRUCTURE OF THE CHITIN DEACETYLASE FROM THE FUNGAL PATHOGEN COLLETOTRICHUM LINDEMUTHIANUM
Overview
The fungal pathogen Colletotrichum lindemuthianum secretes an endo-chitin, de-N-acetylase (ClCDA) to modify exposed hyphal chitin during penetration, and infection of plants. Although a significant amount of biochemical data, is available on fungal chitin de-N-acetylases, no structural data exist., Here we describe the 1.8 A crystal structure of a ClCDA product complex, and the analysis of the reaction mechanism using Hammett linear free, energy relationships, subsite probing, and atomic absorption spectroscopy, studies. The structural data in combination with biochemical data reveal, that ClCDA consists of a single domain encompassing a mononuclear, metalloenzyme which employs a conserved His-His-Asp zinc-binding triad, closely associated with the conserved catalytic base (aspartic acid) ... [(full description)]
About this Structure
2IW0 is a [Single protein] structure of sequence from [Glomerella lindemuthiana] with ZN, ACT, CL and PO4 as [ligands]. Active as [Chitin deacetylase], with EC number [3.5.1.41]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Structure and mechanism of chitin deacetylase from the fungal pathogen Colletotrichum lindemuthianum., Blair DE, Hekmat O, Schuttelkopf AW, Shrestha B, Tokuyasu K, Withers SG, van Aalten DM, Biochemistry. 2006 Aug 8;45(31):9416-26. PMID:16878976
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