2nnw
From Proteopedia
(New page: 200px<br /><applet load="2nnw" size="450" color="white" frame="true" align="right" spinBox="true" caption="2nnw, resolution 2.7Å" /> '''Alternative conformat...) |
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| - | [[Image:2nnw.jpg|left|200px]]<br /><applet load="2nnw" size=" | + | [[Image:2nnw.jpg|left|200px]]<br /><applet load="2nnw" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2nnw, resolution 2.7Å" /> | caption="2nnw, resolution 2.7Å" /> | ||
'''Alternative conformations of Nop56/58-fibrillarin complex and implication for induced-fit assenly of box C/D RNPs'''<br /> | '''Alternative conformations of Nop56/58-fibrillarin complex and implication for induced-fit assenly of box C/D RNPs'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2NNW is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http:// | + | 2NNW is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NNW OCA]. |
==Reference== | ==Reference== | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:01:17 2008'' |
Revision as of 12:01, 23 January 2008
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Alternative conformations of Nop56/58-fibrillarin complex and implication for induced-fit assenly of box C/D RNPs
Overview
The Nop56/58-fibrillarin heterocomplex is a core protein complex of the, box C/D ribonucleoprotein particles that modify and process ribosomal, RNAs. The previous crystal structure of the Archaeoglobus fulgidus complex, revealed a symmetric dimer of two Nop56/58-fibrillarin complexes linked by, the coiled-coil domains of the Nop56/68 proteins. However, because the A., fulgidus Nop56/58 protein lacks some domains found in most other species, it was thought that the bipartite architecture of the heterocomplex was, not likely a general phenomenon. Here we report the crystal structure of, the Nop56/58-fibrillarin complex bound with methylation cofactor, S-adenosyl-L-methionine from Pyrococcus furiosus, at 2.7 A. The new, complex confirms the generality of the previously observed bipartite, arrangement. In addition however, the conformation of Nop56/58 in the new, structure differs substantially from that in the earlier structure. The, distinct conformations of Nop56/58 suggest potential flexibility in, Nop56/58. Computational normal mode analysis supports this view., Importantly, fibrillarin is repositioned within the two complexes. We, propose that hinge motion within Nop56/58 has important implications for, the possibility of simultaneously positioning two catalytic sites at the, two target sites of a bipartite box C/D guide RNA.
About this Structure
2NNW is a Protein complex structure of sequences from Pyrococcus furiosus. Full crystallographic information is available from OCA.
Reference
Alternative Conformations of the Archaeal Nop56/58-Fibrillarin Complex Imply Flexibility in Box C/D RNPs., Oruganti S, Zhang Y, Li H, Robinson H, Terns MP, Terns RM, Yang W, Li H, J Mol Biol. 2007 Aug 31;371(5):1141-50. Epub 2007 Jun 15. PMID:17617422
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