2ow2

From Proteopedia

Revision as of 12:07, 23 January 2008

MMP-9 active site mutant with difluoro butanoic acid inhibitor

Overview

Human matrix metalloproteinase 9 (MMP-9), also called gelatinase B, is, particularly involved in inflammatory processes, bone remodelling and, wound healing, but is also implicated in pathological processes such as, rheumatoid arthritis, atherosclerosis, tumour growth, and metastasis. We, have prepared the inactive E402Q mutant of the truncated catalytic domain, of human MMP-9 and co-crystallized it with active site-directed synthetic, inhibitors of different binding types. Here, we present the X-ray, structures of five MMP-9 complexes with gelatinase-specific, tight binding, inhibitors: a phosphinic acid (AM-409), a pyrimidine-2,4,6-trione, (RO-206-0222), two carboxylate (An-1 and MJ-24), and a trifluoromethyl, hydroxamic acid inhibitor (MS-560). These compounds bind by making a, compromise between optimal coordination of the catalytic zinc, favourable, hydrogen bond formation in the active-site cleft, and accommodation of, their large hydrophobic P1' groups in the slightly flexible S1' cavity, which exhibits distinct rotational conformations of the Pro421 carbonyl, group in each complex. In all these structures, the side-chain of Arg424, located at the bottom of the S1' cavity is not defined in the electron, density beyond C(gamma), indicating its mobility. However, we suggest that, the mobile Arg424 side-chain partially blocks the S1' cavity, which might, explain the weaker binding of most inhibitors with a long P1' side-chain, for MMP-9 compared with the closely related MMP-2 (gelatinase A), which, exhibits a short threonine side-chain at the equivalent position. These, novel structural details should facilitate the design of more selective, MMP-9 inhibitors.

About this Structure

2OW2 is a Single protein structure of sequence from Homo sapiens with , , and as ligands. Active as Gelatinase B, with EC number 3.4.24.35 Full crystallographic information is available from OCA.

Reference

Crystal Structures of MMP-9 Complexes with Five Inhibitors: Contribution of the Flexible Arg424 Side-chain to Selectivity., Tochowicz A, Maskos K, Huber R, Oltenfreiter R, Dive V, Yiotakis A, Zanda M, Bode W, Goettig P, J Mol Biol. 2007 Aug 24;371(4):989-1006. Epub 2007 May 31. PMID:17599356

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