1dwq
From Proteopedia
(New page: 200px<br /> <applet load="1dwq" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dwq, resolution 2.2Å" /> '''CRYSTAL STRUCTURE OF...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1DWQ is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Manihot_esculenta Manihot esculenta]] with ATO as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.37 4.2.1.37]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DWQ OCA]]. | + | 1DWQ is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Manihot_esculenta Manihot esculenta]] with ATO as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Transferred_entry:_3.3.2.4 Transferred entry: 3.3.2.4]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.37 4.2.1.37]]. Structure known Active Sites: ASA and ASB. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DWQ OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: Manihot esculenta]] | [[Category: Manihot esculenta]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| + | [[Category: Transferred entry: 3.3.2.4]] | ||
[[Category: Effenberger, F.]] | [[Category: Effenberger, F.]] | ||
[[Category: Forster, S.]] | [[Category: Forster, S.]] | ||
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[[Category: hydroxynitrile lyase]] | [[Category: hydroxynitrile lyase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:39:49 2007'' |
Revision as of 09:35, 30 October 2007
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CRYSTAL STRUCTURE OF HYDROXYNITRILE LYASE FROM MANIHOT ESCULENTA IN COMPLEX WITH SUBSTRATES ACETONE AND CHLOROACETONE:IMPLICATIONS FOR THE MECHANISM OF CYANOGENESIS
Overview
The crystal structures of hydroxynitrile lyase from Manihot esculenta, (MeHNL) complexed with the native substrate acetone and substrate analogue, chloroacetone have been determined and refined at 2.2 A resolution. The, substrates are positioned in the active site by hydrogen-bond interactions, of the carbonyl O atom with Thr11 OG, Ser80 OG and, to a lesser extent, Cys81 SG. These studies support a mechanism for cyanogenesis as well as, for the stereospecific MeHNL-catalyzed formation of (S)-cyanohydrins, which closely resembles the base-catalyzed chemical reaction of HCN with, carbonyl compounds.
About this Structure
1DWQ is a [Single protein] structure of sequence from [Manihot esculenta] with ATO as [ligand]. Active as [Transferred entry: 3.3.2.4], with EC number [4.2.1.37]. Structure known Active Sites: ASA and ASB. Full crystallographic information is available from [OCA].
Reference
Structure of hydroxynitrile lyase from Manihot esculenta in complex with substrates acetone and chloroacetone: implications for the mechanism of cyanogenesis., Lauble H, Forster S, Miehlich B, Wajant H, Effenberger F, Acta Crystallogr D Biol Crystallogr. 2001 Feb;57(Pt 2):194-200. PMID:11173464
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