2nuo
From Proteopedia
(New page: 200px<br /> <applet load="2nuo" size="450" color="white" frame="true" align="right" spinBox="true" caption="2nuo, resolution 1.50Å" /> '''Crystal structure o...) |
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| - | [[Image:2nuo. | + | [[Image:2nuo.jpg|left|200px]]<br /><applet load="2nuo" size="350" color="white" frame="true" align="right" spinBox="true" |
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caption="2nuo, resolution 1.50Å" /> | caption="2nuo, resolution 1.50Å" /> | ||
'''Crystal structure of a complex of griffithsin with glucose'''<br /> | '''Crystal structure of a complex of griffithsin with glucose'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2NUO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Griffithsia Griffithsia] with GLC, SO4 and EDO as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 2NUO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Griffithsia Griffithsia] with <scene name='pdbligand=GLC:'>GLC</scene>, <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=EDO:'>EDO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NUO OCA]. |
==Reference== | ==Reference== | ||
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[[Category: sugar binding protein]] | [[Category: sugar binding protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:16:48 2008'' |
Revision as of 12:16, 23 January 2008
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Crystal structure of a complex of griffithsin with glucose
Overview
Crystal structures of complexes of an antiviral lectin griffithsin (GRFT), with glucose and N-acetylglucosamine were solved and refined at high, resolution. In both complexes, all six monosaccharide-binding sites of, GRFT were occupied and the mode of binding was similar to that of mannose., In our previous attempts to obtain a complex with N-acetylglucosamine by, soaking, only a single site was occupied; thus, cocrystallization was, clearly superior despite lower concentration of the ligand. Isothermal, titration calorimetric experiments with N-acetylglucosamine, glucose, and, mannose provided enthalpic evidence of distinct binding differences, between the three monosaccharides. A comparison of the mode of binding of, different monosaccharides is discussed in the context of the antiviral, activity of GRFT, based on specific binding to high-mannose-containing, complex carbohydrates found on viral envelopes.
About this Structure
2NUO is a Single protein structure of sequence from Griffithsia with , and as ligands. Full crystallographic information is available from OCA.
Reference
Crystallographic studies of the complexes of antiviral protein griffithsin with glucose and N-acetylglucosamine., Ziolkowska NE, Shenoy SR, O'Keefe BR, Wlodawer A, Protein Sci. 2007 Jul;16(7):1485-9. Epub 2007 Jun 13. PMID:17567736
Page seeded by OCA on Wed Jan 23 14:16:48 2008
Categories: Griffithsia | Single protein | Wlodawer, A. | Ziolkowska, N.E. | EDO | GLC | SO4 | Domain swapping | Griffithsin | Hiv | Lectins | Mannose binding | Sars | Sugar binding protein
