2pnh
From Proteopedia
(New page: 200px<br /><applet load="2pnh" size="450" color="white" frame="true" align="right" spinBox="true" caption="2pnh, resolution 2.250Å" /> '''Escherichia coli Pr...) |
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| - | [[Image:2pnh.jpg|left|200px]]<br /><applet load="2pnh" size=" | + | [[Image:2pnh.jpg|left|200px]]<br /><applet load="2pnh" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2pnh, resolution 2.250Å" /> | caption="2pnh, resolution 2.250Å" /> | ||
'''Escherichia coli PriB E39A variant'''<br /> | '''Escherichia coli PriB E39A variant'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2PNH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | + | 2PNH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PNH OCA]. |
==Reference== | ==Reference== | ||
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[[Category: ob fold]] | [[Category: ob fold]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:17:20 2008'' |
Revision as of 12:17, 23 January 2008
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Escherichia coli PriB E39A variant
Overview
Collapsed DNA replication forks must be reactivated through, origin-independent reloading of the replication machinery (replisome) to, ensure complete duplication of cellular genomes. In E. coli, the, PriA-dependent pathway is the major replication restart mechanism and, requires primosome proteins PriA, PriB, and DnaT for replisome reloading., However, the molecular mechanisms that regulate origin-independent, replisome loading are not fully understood. Here, we demonstrate that, assembly of primosome protein complexes represents a key regulatory, mechanism, as inherently weak PriA-PriB and PriB-DnaT interactions are, strongly stimulated by single-stranded DNA. Furthermore, the binding site, on PriB for single-stranded DNA partially overlaps the binding sites for, PriA and DnaT, suggesting a dynamic primosome assembly process in which, single-stranded DNA is handed off from one primosome protein to another as, a repaired replication fork is reactivated. This model helps explain how, origin-independent initiation of DNA replication is restricted to repaired, replication forks, preventing overreplication of the genome.
About this Structure
2PNH is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
A hand-off mechanism for primosome assembly in replication restart., Lopper M, Boonsombat R, Sandler SJ, Keck JL, Mol Cell. 2007 Jun 22;26(6):781-93. PMID:17588514
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