2uv1
From Proteopedia
(New page: 200px<br /><applet load="2uv1" size="450" color="white" frame="true" align="right" spinBox="true" caption="2uv1, resolution 2.60Å" /> '''HEXAGONAL CRYSTAL FO...) |
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| - | [[Image:2uv1.gif|left|200px]]<br /><applet load="2uv1" size=" | + | [[Image:2uv1.gif|left|200px]]<br /><applet load="2uv1" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2uv1, resolution 2.60Å" /> | caption="2uv1, resolution 2.60Å" /> | ||
'''HEXAGONAL CRYSTAL FORM OF GAMS FROM BACTERIOPHAGE LAMBDA.'''<br /> | '''HEXAGONAL CRYSTAL FORM OF GAMS FROM BACTERIOPHAGE LAMBDA.'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2UV1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http:// | + | 2UV1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UV1 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: putative dna mimetic]] | [[Category: putative dna mimetic]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:23:38 2008'' |
Revision as of 12:23, 23 January 2008
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HEXAGONAL CRYSTAL FORM OF GAMS FROM BACTERIOPHAGE LAMBDA.
Overview
In Escherichia coli, RecBCD processes double-stranded DNA breaks during, the initial stages of homologous recombination. RecBCD contains helicase, and nuclease activities, and unwinds and digests the blunt-ended DNA until, a specific eight-nucleotide sequence, Chi, is encountered. Chi modulates, the nuclease activity of RecBCD and results in a resected DNA end, which, is a substrate for RecA during subsequent steps in recombination. RecBCD, also acts as a defence mechanism against bacteriophage infection by, digesting linear viral DNA present during virus replication or resulting, from the action of restriction endonucleases. To avoid this fate, bacteriophage lambda encodes the gene Gam whose product is an inhibitor of, RecBCD. Gam has been shown to bind to RecBCD and inhibit its helicase and, nuclease activities. We show that Gam inhibits RecBCD by preventing it, from binding DNA. We have solved the crystal structure of Gam from two, different crystal forms. Using the published crystal structure of RecBCD, in complex with DNA we suggest models for the molecular mechanism of, Gam-mediated inhibition of RecBCD. We also propose that Gam could be a, mimetic of single-stranded, and perhaps also double-stranded, DNA.
About this Structure
2UV1 is a Single protein structure of sequence from Enterobacteria phage lambda. Full crystallographic information is available from OCA.
Reference
The Crystal Structure of lambda-Gam Protein Suggests a Model for RecBCD Inhibition., Court R, Cook N, Saikrishnan K, Wigley D, J Mol Biol. 2007 Aug 3;371(1):25-33. Epub 2007 May 18. PMID:17544443
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