2obe
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(New page: 200px<br /><applet load="2obe" size="350" color="white" frame="true" align="right" spinBox="true" caption="2obe, resolution 2.100Å" /> '''Crystal Structure o...)
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Revision as of 12:25, 23 January 2008
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Crystal Structure of Chimpanzee Adenovirus (Type 68/Simian 25) Major Coat Protein Hexon
Overview
Virus-specific neutralizing antibodies present an obstacle to the, effective use of adenovirus vectors for gene therapy and vaccination. The, specific sites recognized by neutralizing antibodies have not been, identified for any adenovirus, but they have been proposed to reside, within the hexon, in small regions of the molecule that are exposed on the, capsid surface and possess sequences that vary among serotypes. We have, mapped the epitopes recognized by a panel of seven hexon-specific, monoclonal antibodies that neutralize the chimpanzee adenovirus 68, (AdC68). Surface plasmon resonance experiments revealed that the, antibodies compete for a single hexon binding site, and experiments with, synthetic peptides indicated that this site resides within just one small, surface loop. Mutations within this loop (but not in other surface loops), permitted virus to escape neutralization by all seven monoclonal, antibodies and to resist neutralization by polyclonal antisera obtained, from animals immunized against AdC68. These results indicate that a single, small surface loop defines a major neutralization site for AdC68 hexon.
About this Structure
2OBE is a Single protein structure of sequence from Simian adenovirus 7 with and as ligands. Full crystallographic information is available from OCA.
Reference
Structure-based identification of a major neutralizing site in an adenovirus hexon., Pichla-Gollon SL, Drinker M, Zhou X, Xue F, Rux JJ, Gao GP, Wilson JM, Ertl HC, Burnett RM, Bergelson JM, J Virol. 2007 Feb;81(4):1680-9. Epub 2006 Nov 15. PMID:17108028
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