2cvr

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[[Image:2cvr.gif|left|200px]]
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[[Image:2cvr.png|left|200px]]
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{{STRUCTURE_2cvr| PDB=2cvr | SCENE= }}
{{STRUCTURE_2cvr| PDB=2cvr | SCENE= }}
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'''NMR solution structure of sso7d mutant, K12L, 12 conformers'''
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===NMR solution structure of sso7d mutant, K12L, 12 conformers===
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==Overview==
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Sso7d is a basic 7-kDa DNA-binding protein from Sulfolobus solfataricus, also endowed with ribonuclease activity. The protein consists of a double-stranded antiparallel beta-sheet, onto which an orthogonal triple-stranded antiparallel beta-sheet is packed, and of a small helical stretch at the C-terminus. Furthermore, the two beta-sheets enclose an aromatic cluster displaying a fishbone geometry. We previously cloned the Sso7d-encoding gene, expressed it in Escherichia coli, and produced several single-point mutants, either of residues located in the hydrophobic core or of Trp23, which is exposed to the solvent and plays a major role in DNA binding. The mutation F31A was dramatically destabilizing, with a loss in thermo- and piezostabilities by at least 27 K and 10 kbar, respectively. Here, we report the solution structure of the F31A mutant, which was determined by NMR spectroscopy using 744 distance constraints obtained from analysis of multidimensional spectra in conjunction with simulated annealing protocols. The most remarkable finding is the change in orientation of the Trp23 side chain, which in the wild type is completely exposed to the solvent, whereas in the mutant is largely buried in the aromatic cluster. This prevents the formation of a cavity in the hydrophobic core of the mutant, which would arise in the absence of structural rearrangements. We found additional changes produced by the mutation, notably a strong distortion in the beta-sheets with loss in several hydrogen bonds, increased flexibility of some stretches of the backbone, and some local strains. On one hand, these features may justify the dramatic destabilization provoked by the mutation; on the other hand, they highlight the crucial role of the hydrophobic core in protein stability. To the best of our knowledge, no similar rearrangement has been so far described as a result of a single-point mutation.
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The line below this paragraph, {{ABSTRACT_PUBMED_10504241}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 10504241 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10504241}}
==About this Structure==
==About this Structure==
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2CVR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CVR OCA].
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[[2cvr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CVR OCA].
==Reference==
==Reference==
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A single-point mutation in the extreme heat- and pressure-resistant sso7d protein from sulfolobus solfataricus leads to a major rearrangement of the hydrophobic core., Consonni R, Santomo L, Fusi P, Tortora P, Zetta L, Biochemistry. 1999 Sep 28;38(39):12709-17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10504241 10504241]
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<ref group="xtra">PMID:10504241</ref><ref group="xtra">PMID:12578354</ref><references group="xtra"/>
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[[Category: Single protein]]
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[[Category: Sulfolobus solfataricus]]
[[Category: Sulfolobus solfataricus]]
[[Category: Alberti, E.]]
[[Category: Alberti, E.]]
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[[Category: Recca, T.]]
[[Category: Recca, T.]]
[[Category: Zetta, L.]]
[[Category: Zetta, L.]]
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[[Category: Dna binding protein]]
[[Category: Dna-binding protein]]
[[Category: Dna-binding protein]]
[[Category: Nmr]]
[[Category: Nmr]]
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[[Category: Sulfolobus solfataricus]]
[[Category: Sulfolobus solfataricus]]
[[Category: Thermostable protein]]
[[Category: Thermostable protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 23:09:58 2008''
 

Revision as of 20:34, 14 March 2011

Image:2cvr.png

Template:STRUCTURE 2cvr

NMR solution structure of sso7d mutant, K12L, 12 conformers

Template:ABSTRACT PUBMED 10504241

About this Structure

2cvr is a 1 chain structure with sequence from Sulfolobus solfataricus. Full experimental information is available from OCA.

Reference

  • Consonni R, Santomo L, Fusi P, Tortora P, Zetta L. A single-point mutation in the extreme heat- and pressure-resistant sso7d protein from sulfolobus solfataricus leads to a major rearrangement of the hydrophobic core. Biochemistry. 1999 Sep 28;38(39):12709-17. PMID:10504241
  • Consonni R, Arosio I, Belloni B, Fogolari F, Fusi P, Shehi E, Zetta L. Investigations of Sso7d catalytic residues by NMR titration shifts and electrostatic calculations. Biochemistry. 2003 Feb 18;42(6):1421-9. PMID:12578354 doi:10.1021/bi0265168

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