2orl
From Proteopedia
(New page: 200px<br /><applet load="2orl" size="450" color="white" frame="true" align="right" spinBox="true" caption="2orl" /> '''Solution structure of the cytochrome c- para...) |
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| - | [[Image:2orl.gif|left|200px]]<br /><applet load="2orl" size=" | + | [[Image:2orl.gif|left|200px]]<br /><applet load="2orl" size="350" color="white" frame="true" align="right" spinBox="true" |
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'''Solution structure of the cytochrome c- para-aminophenol adduct'''<br /> | '''Solution structure of the cytochrome c- para-aminophenol adduct'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2ORL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with HEC and 4NL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 2ORL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=HEC:'>HEC</scene> and <scene name='pdbligand=4NL:'>4NL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ORL OCA]. |
==Reference== | ==Reference== | ||
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[[Category: protein-ligand adduct]] | [[Category: protein-ligand adduct]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:28:53 2008'' |
Revision as of 12:28, 23 January 2008
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Solution structure of the cytochrome c- para-aminophenol adduct
Overview
Protein-protein interactions are driven by specific properties of the, molecular surfaces. Cytochrome c, a small electron transfer protein, is, involved in a number of biologically relevant interactions with, macromolecular partners. Small molecules may interfere with such, interactions by binding to the surface of cytochrome c. Here we, investigated the possibility of weak intermolecular interactions between, reduced cytochrome c and a library of 325 small molecules, using, WaterLOGSY NMR spectroscopy. Specific binding was found for p-aminophenol., The solution structure of the p-aminophenol-cytochrome c adduct was, determined using a combination of in silico tools and NMR-based, restraints. The ligand interacts in a specific binding site on the protein, surface through a combination of stacking and H-bond interactions. Small, but meaningful rearrangements of the solvent-exposed side chains are, observed upon ligand binding and contribute to the stabilization of the, complex.
About this Structure
2ORL is a Single protein structure of sequence from Saccharomyces cerevisiae with and as ligands. Full crystallographic information is available from OCA.
Reference
Cytochrome c and organic molecules: solution structure of the p-aminophenol adduct., Assfalg M, Bertini I, Del Conte R, Giachetti A, Turano P, Biochemistry. 2007 May 29;46(21):6232-8. Epub 2007 May 9. PMID:17488096
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