2pff
From Proteopedia
(New page: 200px<br /><applet load="2pff" size="450" color="white" frame="true" align="right" spinBox="true" caption="2pff, resolution 4.0Å" /> '''Structural Insights o...) |
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| - | [[Image:2pff.jpg|left|200px]]<br /><applet load="2pff" size=" | + | [[Image:2pff.jpg|left|200px]]<br /><applet load="2pff" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2pff, resolution 4.0Å" /> | caption="2pff, resolution 4.0Å" /> | ||
'''Structural Insights of Yeast Fatty Acid Synthase'''<br /> | '''Structural Insights of Yeast Fatty Acid Synthase'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2PFF is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Active as [http://en.wikipedia.org/wiki/Fatty-acyl-CoA_synthase Fatty-acyl-CoA synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.86 2.3.1.86] Full crystallographic information is available from [http:// | + | 2PFF is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Active as [http://en.wikipedia.org/wiki/Fatty-acyl-CoA_synthase Fatty-acyl-CoA synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.86 2.3.1.86] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PFF OCA]. |
==Reference== | ==Reference== | ||
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[[Category: malonyltransferase]] | [[Category: malonyltransferase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:29:32 2008'' |
Revision as of 12:29, 23 January 2008
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Structural Insights of Yeast Fatty Acid Synthase
Overview
In yeast, the whole metabolic pathway for making 16- and 18-carbon fatty, acids is carried out by fatty acid synthase, a 2.6 megadalton, molecular-weight macromolecular assembly containing six copies of all, eight catalytic centers. We have determined its crystal structure, which, illuminates how this enzyme is initially activated and then carries out, multiple steps of synthesis in each of six sterically isolated reaction, chambers. Six of the catalytic sites are in the wall of the assembly, facing an acyl carrier protein (ACP) bound to the ketoacyl synthase, domain. Two-dimensional diffusion of substrates to the catalytic sites may, be achieved by the electrostatically negative ACP swinging to each of the, six electrostatically positive catalytic sites. The phosphopantetheinyl, transferase domain lies outside the shell of the assembly, inaccessible to, ACP that lies inside, suggesting that the attachment of the pantetheine, arm to ACP must occur before complete assembly of the complex.
About this Structure
2PFF is a Protein complex structure of sequences from Saccharomyces cerevisiae. Active as Fatty-acyl-CoA synthase, with EC number 2.3.1.86 Full crystallographic information is available from OCA.
Reference
The crystal structure of yeast fatty acid synthase, a cellular machine with eight active sites working together., Lomakin IB, Xiong Y, Steitz TA, Cell. 2007 Apr 20;129(2):319-32. PMID:17448991[[Category: enoyl-[acyl-carrier-protein] reductase]]
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