2v2k
From Proteopedia
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| - | [[Image:2v2k.jpg|left|200px]]<br /><applet load="2v2k" size=" | + | [[Image:2v2k.jpg|left|200px]]<br /><applet load="2v2k" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2v2k, resolution 1.60Å" /> | caption="2v2k, resolution 1.60Å" /> | ||
'''THE CRYSTAL STRUCTURE OF FDXA, A 7FE FERREDOXIN FROM MYCOBACTERIUM SMEGMATIS'''<br /> | '''THE CRYSTAL STRUCTURE OF FDXA, A 7FE FERREDOXIN FROM MYCOBACTERIUM SMEGMATIS'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2V2K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_smegmatis Mycobacterium smegmatis] with ACT and F3S as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:F3s Binding Site For Chain B'>AC1</scene>. Full crystallographic information is available from [http:// | + | 2V2K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_smegmatis Mycobacterium smegmatis] with <scene name='pdbligand=ACT:'>ACT</scene> and <scene name='pdbligand=F3S:'>F3S</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:F3s Binding Site For Chain B'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V2K OCA]. |
==Reference== | ==Reference== | ||
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[[Category: transport]] | [[Category: transport]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:34:35 2008'' |
Revision as of 12:34, 23 January 2008
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THE CRYSTAL STRUCTURE OF FDXA, A 7FE FERREDOXIN FROM MYCOBACTERIUM SMEGMATIS
Overview
Mycobacterium smegmatis ferredoxin FdxA, which has an orthologue, ferredoxin in Mycobacterium tuberculosis, FdxC, contains both one [3Fe-4S], and one [4Fe-4S] cluster. M. smegmatis FdxA has been shown to be a, preferred ferredoxin substrate of FprA [F. Fischer, D. Raimondi, A., Aliverti, G. Zanetti, Mycobacterium tuberculosis FprA, a novel bacterial, NADPH-ferredoxin reductase, Eur. J. Biochem. 269 (2002) 3005-3013], an, adrenodoxin reductase-like flavoprotein of M. tuberculosis, suggesting, that M. tuberculosis FdxC could be the physiological partner of the enzyme, in providing reducing power to the cytochromes P450. We report here the, crystal structure of FdxA at 1.6A resolution (R(factor) 16.5%, R(free), 20.2%). Besides providing an insight on protein architecture for this, 106-residue ferredoxin, our crystallographic investigation highlights, lability of the [4Fe-4S] center, which is shown to loose a Fe atom during, crystal growth. Due to their high similarity (87% sequence identity), the, structure here reported can be considered a valuable model for M., tuberculosis FdxC, thus representing a step forward in the study of the, complex mycobacterial redox pathways.
About this Structure
2V2K is a Single protein structure of sequence from Mycobacterium smegmatis with and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
The crystal structure of FdxA, a 7Fe ferredoxin from Mycobacterium smegmatis., Ricagno S, de Rosa M, Aliverti A, Zanetti G, Bolognesi M, Biochem Biophys Res Commun. 2007 Aug 17;360(1):97-102. Epub 2007 Jun 12. PMID:17577575
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Categories: Mycobacterium smegmatis | Single protein | Aliverti, A. | Bolognesi, M. | Ricagno, S. | Rosa, M.De. | Zanetti, G. | ACT | F3S | 3fe-4s | 4fe-4s | 4fe-4s cluster instability | 7fe ferredoxin | Electron transfer | Electron transport | Fe-s cluster | Iron | Iron-sulfur | Metal-binding | Mycobacterium tuberculosis | Transport
