2pak
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(New page: 200px<br /><applet load="2pak" size="350" color="white" frame="true" align="right" spinBox="true" caption="2pak, resolution 2.40Å" /> '''Structure of a H51N ...)
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Revision as of 12:38, 23 January 2008
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Structure of a H51N mutant dTDP-4-keto-6-deoxy-D-glucose-3,4-ketoisomerase from Aneurinibacillus thermoaerophilus complexed with TDP
Overview
The repeating unit of the glycan chain in the S-layer of the bacterium, Aneurinibacillus thermoaerophilus L420-91(T) is composed of four, alpha-d-rhamnose molecules and two, 3-acetamido-3,6-dideoxy-alpha-d-galactose moieties (abbreviated as, Fucp3NAc). Formation of the glycan layer requires nucleotide-activated, sugars as the donor molecules. Whereas the enzymes involved in the, synthesis of GDP-rhamnose have been well characterized, less is known, regarding the structures and enzymatic mechanisms of the enzymes required, for the production of dTDP-Fucp3NAc. One of the enzymes involved in the, biosynthesis of dTDP-Fucp3NAc is a 3,4-ketoisomerase, hereafter referred, to as FdtA. Here we describe the first three-dimensional structure of this, sugar isomerase complexed with dTDP and solved to 1.5 A resolution. The, FdtA dimer assumes an almost jellyfish-like appearance with the sole, alpha-helices representing the tentacles. Formation of the FdtA dimer, represents a classical example of domain swapping whereby beta-strands 2, and 3 from one subunit form part of a beta-sheet in the second subunit., The active site architecture of FdtA is characterized by a cluster of, three histidine residues, two of which, His(49) and His(51), appear to be, strictly conserved in the amino acid sequences deposited to date., Site-directed mutagenesis experiments, enzymatic assays, and x-ray, crystallographic analyses suggest that His(49) functions as an active site, base.
About this Structure
2PAK is a Single protein structure of sequence from Aneurinibacillus thermoaerophilus with as ligand. Full crystallographic information is available from OCA.
Reference
The X-ray Structure of dTDP-4-Keto-6-deoxy-D-glucose-3,4-ketoisomerase., Davis ML, Thoden JB, Holden HM, J Biol Chem. 2007 Jun 29;282(26):19227-36. Epub 2007 Apr 25. PMID:17459872
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