1a47
From Proteopedia
(New page: 200px<br /> <applet load="1a47" size="450" color="white" frame="true" align="right" spinBox="true" caption="1a47, resolution 2.56Å" /> '''CGTASE FROM THERMOA...) |
|||
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1A47 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Thermoanaerobacterium_thermosulfurigenes Thermoanaerobacterium thermosulfurigenes]] with CA as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.19 2.4.1.19]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1A47 OCA]]. | + | 1A47 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Thermoanaerobacterium_thermosulfurigenes Thermoanaerobacterium thermosulfurigenes]] with CA as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Cyclomaltodextrin_glucanotransferase Cyclomaltodextrin glucanotransferase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.19 2.4.1.19]]. Structure known Active Sites: AM1, AM2, AM3, AP1, AP2, AP3, BS3, CA1 and CA2. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1A47 OCA]]. |
==Reference== | ==Reference== | ||
Engineering of cyclodextrin product specificity and pH optima of the thermostable cyclodextrin glycosyltransferase from Thermoanaerobacterium thermosulfurigenes EM1., Wind RD, Uitdehaag JC, Buitelaar RM, Dijkstra BW, Dijkhuizen L, J Biol Chem. 1998 Mar 6;273(10):5771-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9488711 9488711] | Engineering of cyclodextrin product specificity and pH optima of the thermostable cyclodextrin glycosyltransferase from Thermoanaerobacterium thermosulfurigenes EM1., Wind RD, Uitdehaag JC, Buitelaar RM, Dijkstra BW, Dijkhuizen L, J Biol Chem. 1998 Mar 6;273(10):5771-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9488711 9488711] | ||
| + | [[Category: Cyclomaltodextrin glucanotransferase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Thermoanaerobacterium thermosulfurigenes]] | [[Category: Thermoanaerobacterium thermosulfurigenes]] | ||
| Line 26: | Line 27: | ||
[[Category: thermostable]] | [[Category: thermostable]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:45:00 2007'' |
Revision as of 09:40, 30 October 2007
|
CGTASE FROM THERMOANAEROBACTERIUM THERMOSULFURIGENES EM1 IN COMPLEX WITH A MALTOHEXAOSE INHIBITOR
Overview
The product specificity and pH optimum of the thermostable cyclodextrin, glycosyltransferase (CGTase) from Thermoanaerobacterium thermosulfurigenes, EM1 was engineered using a combination of x-ray crystallography and, site-directed mutagenesis. Previously, a crystal soaking experiment with, the Bacillus circulans strain 251 beta-CGTase had revealed a maltononaose, inhibitor bound to the enzyme in an extended conformation. An identical, experiment with the CGTase from T. thermosulfurigenes EM1 resulted in a, 2.6-A resolution x-ray structure of a complex with a maltohexaose, inhibitor, bound in a different conformation. We hypothesize that the new, maltohexaose conformation is related to the enhanced alpha-cyclodextrin, production of the CGTase. The detailed structural information ... [(full description)]
About this Structure
1A47 is a [Single protein] structure of sequence from [Thermoanaerobacterium thermosulfurigenes] with CA as [ligand]. Active as [Cyclomaltodextrin glucanotransferase], with EC number [2.4.1.19]. Structure known Active Sites: AM1, AM2, AM3, AP1, AP2, AP3, BS3, CA1 and CA2. Full crystallographic information is available from [OCA].
Reference
Engineering of cyclodextrin product specificity and pH optima of the thermostable cyclodextrin glycosyltransferase from Thermoanaerobacterium thermosulfurigenes EM1., Wind RD, Uitdehaag JC, Buitelaar RM, Dijkstra BW, Dijkhuizen L, J Biol Chem. 1998 Mar 6;273(10):5771-9. PMID:9488711
Page seeded by OCA on Tue Oct 30 11:45:00 2007
