2cdm
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:2cdm.gif|left|200px]]<br /><applet load="2cdm" size=" | + | [[Image:2cdm.gif|left|200px]]<br /><applet load="2cdm" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2cdm, resolution 2.70Å" /> | caption="2cdm, resolution 2.70Å" /> | ||
'''THE STRUCTURE OF TRWC COMPLEXED WITH A 27-MER DNA COMPRISING THE RECOGNITION HAIRPIN AND THE CLEAVAGE SITE'''<br /> | '''THE STRUCTURE OF TRWC COMPLEXED WITH A 27-MER DNA COMPRISING THE RECOGNITION HAIRPIN AND THE CLEAVAGE SITE'''<br /> | ||
| Line 7: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2CDM is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:So4 Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http:// | + | 2CDM is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:So4 Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CDM OCA]. |
==Reference== | ==Reference== | ||
| Line 27: | Line 27: | ||
[[Category: relaxase]] | [[Category: relaxase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:42:20 2008'' |
Revision as of 12:42, 23 January 2008
|
THE STRUCTURE OF TRWC COMPLEXED WITH A 27-MER DNA COMPRISING THE RECOGNITION HAIRPIN AND THE CLEAVAGE SITE
Overview
TrwC is a DNA strand transferase that catalyzes the initial and final, stages of conjugative DNA transfer. We have solved the crystal structure, of the N-terminal relaxase domain of TrwC in complex with a 27 base-long, DNA oligonucleotide that contains both the recognition hairpin and the, scissile phosphate. In addition, a series of ternary structures of, protein-DNA complexes with different divalent cations at the active site, have been solved. Systematic anomalous difference analysis allowed us to, determine unambiguously the nature of the metal bound. Zn2+, Ni2+ and Cu2+, were found to bind the histidine-triad metal binding site. Comparison of, the structures of the different complexes suggests two pathways for the, DNA to exit the active pocket. They are probably used at different steps, of the conjugative DNA-processing reaction. The structural information, allows us to propose (i) an enzyme mechanism where the scissile phosphate, is polarized by the metal ion facilitating the nucleophilic attack of the, catalytic tyrosine, and (ii) a probable sequence of events during, conjugative DNA processing that explains the biological function of the, relaxase.
About this Structure
2CDM is a Protein complex structure of sequences from Escherichia coli with as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Unveiling the molecular mechanism of a conjugative relaxase: The structure of TrwC complexed with a 27-mer DNA comprising the recognition hairpin and the cleavage site., Boer R, Russi S, Guasch A, Lucas M, Blanco AG, Perez-Luque R, Coll M, de la Cruz F, J Mol Biol. 2006 May 5;358(3):857-69. Epub 2006 Feb 28. PMID:16540117
Page seeded by OCA on Wed Jan 23 14:42:20 2008
