Avidin
From Proteopedia
| Line 20: | Line 20: | ||
== Strepavidin == | == Strepavidin == | ||
| - | [[2bc3]], [[2iza]], [[2izb]], [[2izc]], [[2izd]], [[2ize]], [[2rta]], [[2rtb]], [[2rtc]] - StrAv<br /> | + | [[2bc3]], [[2iza]], [[2izb]], [[2izc]], [[2izd]], [[2ize]], [[2rta]], [[2rtb]], [[2rtc]] - StrAv - ''Streptomyces avidinii''<br /> |
| - | [[1rxh]] – StrAv (mutant)+ biotinyl p-nitroaniline | + | [[1rxh]] – StrAv (mutant)+ biotinyl p-nitroaniline <br /> |
[[1rxj]], [[1rxk]], [[1nqm]], [[1kff]], [[1kl4]], [[1luq]], [[1mm9]], [[1moy]], [[1n4j]], [[1n7y]], [[1n9y]], [[1nbx]], [[1swf]], [[1swu]], [[3mm0]] - StrAv (mutant) <br /> | [[1rxj]], [[1rxk]], [[1nqm]], [[1kff]], [[1kl4]], [[1luq]], [[1mm9]], [[1moy]], [[1n4j]], [[1n7y]], [[1n9y]], [[1nbx]], [[1swf]], [[1swu]], [[3mm0]] - StrAv (mutant) <br /> | ||
[[1kl3]], [[1kl5]] - StrAv (mutant) + strep-tag II peptide<br /> | [[1kl3]], [[1kl5]] - StrAv (mutant) + strep-tag II peptide<br /> | ||
| Line 30: | Line 30: | ||
[[1mk5]], [[1swd]], [[1swe]], [[2f01]], [[2gh7]] - StrAv core+biotin<br /> | [[1mk5]], [[1swd]], [[1swe]], [[2f01]], [[2gh7]] - StrAv core+biotin<br /> | ||
[[1swa]], [[1swb]], [[1swc]] - StrAv core<br /> | [[1swa]], [[1swb]], [[1swc]] - StrAv core<br /> | ||
| - | [[1swh]], [[1swj]], [[1swl]], [[1swo]], [[1swq]], [[1sws]] - StrAv core (mutant)<br /> | + | [[1swh]], [[1swj]], [[1swl]], [[1swo]], [[1swq]], [[1sws]], [[2y3e]] - StrAv core (mutant)<br /> |
| - | [[1swk]], [[1swn]], [[1swp]], [[1swr]], [[1swt]], [[3mg5]] - StrAv core (mutant) + biotin<br /> | + | [[1swk]], [[1swn]], [[1swp]], [[1swr]], [[1swt]], [[3mg5]], [[2y3f]] - StrAv core (mutant) + biotin<br /> |
[[1stp]], [[2izf]], [[2izg]], [[2izh]], [[2izi]], [[2izj]], [[2rtd]], [[2rte]], [[2rtf]], [[2rtg]] - StrAv +biotin | [[1stp]], [[2izf]], [[2izg]], [[2izh]], [[2izi]], [[2izj]], [[2rtd]], [[2rte]], [[2rtf]], [[2rtg]] - StrAv +biotin | ||
[[1hqq]], [[1hxl]] – StrAv + MP-2 (mutant)<br /> | [[1hqq]], [[1hxl]] – StrAv + MP-2 (mutant)<br /> | ||
Revision as of 09:36, 20 March 2011
Template:STRUCTURE 1ave 3D Structure of Avidin
Avidin (Av) is a protein found in egg white which binds biotin (vitamin B7) with high affinity. This property makes it a powerful tool in various protein pull-down assays. Similar proteins are strepavidin (StrAv) from the bacterium Streptomyces avidinii, Rhizavidin (RhiAv) from Rhizobium etli, Xenavidin from frog, and the avidin-related proteins AVR2 and AVR4. The N-acetylglucosamine sugars are shown as space filling objects in the image on the left.
Avidin
3fdc, 2jgs, 2c4i, 1vyo, 1rav, 1ave – cAv – chicken
2a5b, 2a5c, 2a8g – cAv+deoxyribose
1nqn, 2cam – cAv (mutant)
1ldo, 1ldq, 1lel, 1ij8, 1avd, 2avi – cAV + biotin derivative
Strepavidin
2bc3, 2iza, 2izb, 2izc, 2izd, 2ize, 2rta, 2rtb, 2rtc - StrAv - Streptomyces avidinii
1rxh – StrAv (mutant)+ biotinyl p-nitroaniline
1rxj, 1rxk, 1nqm, 1kff, 1kl4, 1luq, 1mm9, 1moy, 1n4j, 1n7y, 1n9y, 1nbx, 1swf, 1swu, 3mm0 - StrAv (mutant)
1kl3, 1kl5 - StrAv (mutant) + strep-tag II peptide
1rst, 1rsu - StrAv + strep-tag II peptide
1df8, 1mep, 1n43, 1n9m, 1ndj, 1swg - StrAv (mutant)+biotin
1nc9 - StrAv (mutant)+iminobiotin
2izl, 2rtl, 2rtm, 2rtn, 2rto, 2rtp, 2rtq, 2rtr - StrAv +iminobiotin
1mk5, 1swd, 1swe, 2f01, 2gh7 - StrAv core+biotin
1swa, 1swb, 1swc - StrAv core
1swh, 1swj, 1swl, 1swo, 1swq, 1sws, 2y3e - StrAv core (mutant)
1swk, 1swn, 1swp, 1swr, 1swt, 3mg5, 2y3f - StrAv core (mutant) + biotin
1stp, 2izf, 2izg, 2izh, 2izi, 2izj, 2rtd, 2rte, 2rtf, 2rtg - StrAv +biotin
1hqq, 1hxl – StrAv + MP-2 (mutant)
1hy2 - StrAv + MP-1
1hxz - StrAv + MP-2
1pts, 1sld, 1sle, 1slg, 1str, 1sts, 1vwa, 1vwb, 1vwc, 1vwd, 1vwe, 1vwf, 1vwg, 1vwh, 1vwi, 1vwj, 1vwk, 1vwl, 1vwm, 1vwn, 1vwo, 1vwp, 1vwq, 1vwr, 2g5l - StrAv + peptide
1slf - StrAv + SO4
1sre, 1srf, 1srg, 1srh, 1sri, 1srj - StrAv + benzoic acid ligands
1lcv, 1lcw, 1lcz, 1i9h – StrAv+biotin derivative
2izk, 2rth, 2rti, 2rtj, 2rtk – StrAV+glycouril
2qcb, 2wpu – StrAv+Ru ligand
Rhizavidin
3ew1 – RhiAv – Rhizobium etli
3ew2 – RhiAV+biotin
Xenavidin
Tamavidin
2zsc – TanAv – Tamogitake mushroom
AVR2
1wbi – cAVR2
AVR4
2fhl – cAVR4+biotinyl p-nitroaniline
2of9, 2ofa, 1y53 - cAVR4 (mutant)
2fhn, 2of8 - cAVR4 (mutant)+biotinyl p-nitroaniline
1y52, 1y55 - cAVR4 (mutant)+biotin
Proteopedia Page Contributors and Editors (what is this?)
Michal Harel, Alexander Berchansky, Joel L. Sussman, Marcin Jozef Suskiewicz, Jaime Prilusky
