2enx
From Proteopedia
(New page: 200px<br /><applet load="2enx" size="450" color="white" frame="true" align="right" spinBox="true" caption="2enx, resolution 2.80Å" /> '''Structure of the fam...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:2enx.jpg|left|200px]]<br /><applet load="2enx" size=" | + | [[Image:2enx.jpg|left|200px]]<br /><applet load="2enx" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2enx, resolution 2.80Å" /> | caption="2enx, resolution 2.80Å" /> | ||
'''Structure of the family II inorganic pyrophosphatase from Streptococcus agalactiae at 2.8 resolution'''<br /> | '''Structure of the family II inorganic pyrophosphatase from Streptococcus agalactiae at 2.8 resolution'''<br /> | ||
| Line 7: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2ENX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_agalactiae Streptococcus agalactiae] with MN, MG, 2PN and TRP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Inorganic_diphosphatase Inorganic diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.1 3.6.1.1] Full crystallographic information is available from [http:// | + | 2ENX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_agalactiae Streptococcus agalactiae] with <scene name='pdbligand=MN:'>MN</scene>, <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=2PN:'>2PN</scene> and <scene name='pdbligand=TRP:'>TRP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Inorganic_diphosphatase Inorganic diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.1 3.6.1.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ENX OCA]. |
==Reference== | ==Reference== | ||
| Line 30: | Line 30: | ||
[[Category: streptococcus agalactiae]] | [[Category: streptococcus agalactiae]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:52:36 2008'' |
Revision as of 12:52, 23 January 2008
|
Structure of the family II inorganic pyrophosphatase from Streptococcus agalactiae at 2.8 resolution
Overview
Streptococcus agalactiae, a prokaryote that causes infections in neonates, and immunocompromised adults, has a serine/threonine protein kinase (STK), signalling cascade. The structure of one of the targets, a family II, inorganic pyrophosphatase, has been solved by molecular replacement and, refined at 2.80 A resolution to an R factor of 19.2% (R(free) = 26.7%)., The two monomers in the asymmetric unit are related by a, noncrystallographic twofold axis, but the biological dimer is formed by a, crystallographic twofold. Each monomer contains the pyrophosphate analogue, imidodiphosphate (PNP) and three metal ions per active site: two Mn(2+), ions in sites M1 and M2 and an Mg(2+) ion in site M3. The enzyme is in the, closed conformation. Like other family II enzymes, the structure consists, of two domains (residues 1-191 and 198-311), with the active site located, between them. The conformation of Lys298 in the active site is different, from those observed previously and it coordinates to the conserved DHH, motif in a unique way. The structure suggests that Ser150, Ser194, Ser195, and Ser296 are the most likely targets for the Ser/Thr kinase and, phosphatase because they are surface-accessible and either in the active, site or in the hinge region between the two domains.
About this Structure
2ENX is a Single protein structure of sequence from Streptococcus agalactiae with , , and as ligands. Active as Inorganic diphosphatase, with EC number 3.6.1.1 Full crystallographic information is available from OCA.
Reference
Structure of the Streptococcus agalactiae family II inorganic pyrophosphatase at 2.80 A resolution., Rantanen MK, Lehtio L, Rajagopal L, Rubens CE, Goldman A, Acta Crystallogr D Biol Crystallogr. 2007 Jun;63(Pt 6):738-43. Epub 2007, May 15. PMID:17505113
Page seeded by OCA on Wed Jan 23 14:52:36 2008
Categories: Inorganic diphosphatase | Single protein | Streptococcus agalactiae | Goldman, A. | Lehtio, L. | Rajagopal, L. | Rantanen, M.K. | Rubens, C.E. | 2PN | MG | MN | TRP | Family ii | Inorganic | Phosphorylation | Pyrophosphatase | Signalling
