2jie
From Proteopedia
(New page: 200px<br /> <applet load="2jie" size="450" color="white" frame="true" align="right" spinBox="true" caption="2jie, resolution 2.3Å" /> '''BETA-GLUCOSIDASE B F...) |
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==About this Structure== | ==About this Structure== | ||
| - | 2JIE is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Paenibacillus_polymyxa Paenibacillus polymyxa]] with G2F as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2JIE OCA]]. | + | 2JIE is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Paenibacillus_polymyxa Paenibacillus polymyxa]] with G2F as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2JIE OCA]]. |
==Reference== | ==Reference== | ||
Crystal Structures of Paenibacillus polymyxa beta-Glucosidase B Complexes Reveal the Molecular Basis of Substrate Specificity and Give New Insights into the Catalytic Machinery of Family I Glycosidases., Isorna P, Polaina J, Latorre-Garcia L, Canada FJ, Gonzalez B, Sanz-Aparicio J, J Mol Biol. 2007 Aug 31;371(5):1204-18. Epub 2007 Jun 2. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17585934 17585934] | Crystal Structures of Paenibacillus polymyxa beta-Glucosidase B Complexes Reveal the Molecular Basis of Substrate Specificity and Give New Insights into the Catalytic Machinery of Family I Glycosidases., Isorna P, Polaina J, Latorre-Garcia L, Canada FJ, Gonzalez B, Sanz-Aparicio J, J Mol Biol. 2007 Aug 31;371(5):1204-18. Epub 2007 Jun 2. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17585934 17585934] | ||
| + | [[Category: Beta-glucosidase]] | ||
[[Category: Paenibacillus polymyxa]] | [[Category: Paenibacillus polymyxa]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: polysaccharide degradation]] | [[Category: polysaccharide degradation]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:48:22 2007'' |
Revision as of 09:43, 30 October 2007
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BETA-GLUCOSIDASE B FROM BACILLUS POLYMYXA COMPLEXED WITH 2-F-GLUCOSE
Overview
Bacteria species involved in degradation of cellulosic substrates produce, a variety of enzymes for processing related compounds along the hydrolytic, pathway. Paenibacillus polymyxa encodes two homologous beta-glucosidases, BglA and BglB, presenting different quaternary structures and substrate, specificities. We previously reported the 3D-structure of BglA, which is, highly specific against cellobiose. Here, we present structural analysis, of BglB, a monomeric enzyme that acts as an exo-beta-glucosidase, hydrolyzing cellobiose and cellodextrins of higher degree of, polymerization. The crystal structure of BglB shows that several polar, residues narrow the active site pocket and contour additional subsites., The structure of the BglB-cellotetraose complex confirms these subsites, ... [(full description)]
About this Structure
2JIE is a [Single protein] structure of sequence from [Paenibacillus polymyxa] with G2F as [ligand]. Active as [Beta-glucosidase], with EC number [3.2.1.21]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Crystal Structures of Paenibacillus polymyxa beta-Glucosidase B Complexes Reveal the Molecular Basis of Substrate Specificity and Give New Insights into the Catalytic Machinery of Family I Glycosidases., Isorna P, Polaina J, Latorre-Garcia L, Canada FJ, Gonzalez B, Sanz-Aparicio J, J Mol Biol. 2007 Aug 31;371(5):1204-18. Epub 2007 Jun 2. PMID:17585934
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