2pe6
From Proteopedia
(New page: 200px<br /> <applet load="2pe6" size="450" color="white" frame="true" align="right" spinBox="true" caption="2pe6, resolution 2.40Å" /> '''Non-covalent comple...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:2pe6.gif|left|200px]]<br /> | + | [[Image:2pe6.gif|left|200px]]<br /><applet load="2pe6" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2pe6" size=" | + | |
caption="2pe6, resolution 2.40Å" /> | caption="2pe6, resolution 2.40Å" /> | ||
'''Non-covalent complex between human SUMO-1 and human Ubc9'''<br /> | '''Non-covalent complex between human SUMO-1 and human Ubc9'''<br /> | ||
| Line 6: | Line 5: | ||
==Overview== | ==Overview== | ||
The SUMO E2 Ubc9 serves as a lynchpin in the SUMO conjugation pathway, interacting with the SUMO E1 during activation, with thioester linked SUMO, after E1 transfer and with the substrate and SUMO E3 ligases during, conjugation. Here, we describe the structure determination of a, non-covalent complex between human Ubc9 and SUMO-1 at 2.4 A resolution., Non-covalent interactions between Ubc9 and SUMO are conserved in human and, yeast insomuch as human Ubc9 interacts with each of the human SUMO, isoforms, and yeast Ubc9 interacts with Smt3, the yeast SUMO ortholog., Structural comparisons reveal similarities to several other non-covalent, complexes in the ubiquitin pathway, suggesting that the non-covalent, Ubc9-SUMO interface may be important for poly-SUMO chain formation, for E2, recruitment to SUMO conjugated substrates, or for mediating E2, interactions with either E1 or E3 ligases. Biochemical analysis suggests, that this surface is less important for E1 activation or di-SUMO-2, formation, but more important for E3 interactions and for poly-SUMO chain, formation when the chain exceeds more than two SUMO proteins. | The SUMO E2 Ubc9 serves as a lynchpin in the SUMO conjugation pathway, interacting with the SUMO E1 during activation, with thioester linked SUMO, after E1 transfer and with the substrate and SUMO E3 ligases during, conjugation. Here, we describe the structure determination of a, non-covalent complex between human Ubc9 and SUMO-1 at 2.4 A resolution., Non-covalent interactions between Ubc9 and SUMO are conserved in human and, yeast insomuch as human Ubc9 interacts with each of the human SUMO, isoforms, and yeast Ubc9 interacts with Smt3, the yeast SUMO ortholog., Structural comparisons reveal similarities to several other non-covalent, complexes in the ubiquitin pathway, suggesting that the non-covalent, Ubc9-SUMO interface may be important for poly-SUMO chain formation, for E2, recruitment to SUMO conjugated substrates, or for mediating E2, interactions with either E1 or E3 ligases. Biochemical analysis suggests, that this surface is less important for E1 activation or di-SUMO-2, formation, but more important for E3 interactions and for poly-SUMO chain, formation when the chain exceeds more than two SUMO proteins. | ||
| - | |||
| - | ==Disease== | ||
| - | Known diseases associated with this structure: Orofacial cleft 10 OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=601912 601912]] | ||
==About this Structure== | ==About this Structure== | ||
| - | 2PE6 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 2PE6 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PE6 OCA]. |
==Reference== | ==Reference== | ||
| Line 25: | Line 21: | ||
[[Category: ubiquitin-like]] | [[Category: ubiquitin-like]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:59:33 2008'' |
Revision as of 12:59, 23 January 2008
|
Non-covalent complex between human SUMO-1 and human Ubc9
Overview
The SUMO E2 Ubc9 serves as a lynchpin in the SUMO conjugation pathway, interacting with the SUMO E1 during activation, with thioester linked SUMO, after E1 transfer and with the substrate and SUMO E3 ligases during, conjugation. Here, we describe the structure determination of a, non-covalent complex between human Ubc9 and SUMO-1 at 2.4 A resolution., Non-covalent interactions between Ubc9 and SUMO are conserved in human and, yeast insomuch as human Ubc9 interacts with each of the human SUMO, isoforms, and yeast Ubc9 interacts with Smt3, the yeast SUMO ortholog., Structural comparisons reveal similarities to several other non-covalent, complexes in the ubiquitin pathway, suggesting that the non-covalent, Ubc9-SUMO interface may be important for poly-SUMO chain formation, for E2, recruitment to SUMO conjugated substrates, or for mediating E2, interactions with either E1 or E3 ligases. Biochemical analysis suggests, that this surface is less important for E1 activation or di-SUMO-2, formation, but more important for E3 interactions and for poly-SUMO chain, formation when the chain exceeds more than two SUMO proteins.
About this Structure
2PE6 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure and Analysis of a Complex between SUMO and Ubc9 Illustrates Features of a Conserved E2-Ubl Interaction., Capili AD, Lima CD, J Mol Biol. 2007 Jun 8;369(3):608-18. Epub 2007 Apr 6. PMID:17466333
Page seeded by OCA on Wed Jan 23 14:59:33 2008
Categories: Homo sapiens | Protein complex | Capili, A.D. | Lima, C.D. | Conjugation | Smt3 | Sumo | Ubc9 | Ubiquitin-like
