Sandbox Reserved 199
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== Introduction == | == Introduction == | ||
Revision as of 04:47, 30 March 2011
| This Sandbox is Reserved from Feb 02, 2011, through Jul 31, 2011 for use by the Biochemistry II class at the Butler University at Indianapolis, IN USA taught by R. Jeremy Johnson. This reservation includes Sandbox Reserved 191 through Sandbox Reserved 200. |
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Contents |
Introduction
NMR Ribonuclease History
In 1957, the first work was published examining the structure of bovine pancreatic Ribonuclease using 1-Dimensional 1H NMR by Martin Saunder et al.
In 1988, Udgaonkar et al. used 2-dimensional 1H NMR to study bovine pancreatic Ribonuclease and examined protein folding dynamics, which supported the framework model protein folding mechanism.
Ribonuclease Folding Dynamics NMR Study
Experimental Prcedure
Using 2-dimensional 1H NMR, Udgaonkar et al. studied the folding pathway of bovine pancreatic Ribonuclease using an exchange reaction between with solvent protons. 2- dimensional 1H NMR allowed for monitoring of proton exchange in the amide backbone for ten second time intervals, and this proton labeling could be terminated via a rapid drop in pH reaction conditions. This research focused on initial protein folding steps.
Starting with denatured wt Ribonuclease, it was suggested that as the peptide began to fold, the backbone amide proteins would become less energetically favorable to exchange protons with the solvent as the backbone amide protons became involved in folding-related intermolecular interactions (such as ).
Data and Results
became evident as those to be involved in folding-related intermolecular interactions during initial protein folding steps: , , , , and . All five of these protons are involved in hydrogen bonding within the of Ribonuclease; therefore, it was believed that this secondary structure was the starting point for the folding mechanism of Ribonuclease. Furthermore, this suggests the formation of a stable secondary structure before the formation of the , which is consistent with the framework model protein folding mechanism (in comparison to the jigsaw puzzle model).
References
External Resources
Daniel Kroupa 05:03, 30 March 2011 (IST)
