2nyt
From Proteopedia
(New page: 200px<br /> <applet load="2nyt" size="450" color="white" frame="true" align="right" spinBox="true" caption="2nyt, resolution 2.5Å" /> '''The APOBEC2 Crystal ...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:2nyt.gif|left|200px]]<br /> | + | [[Image:2nyt.gif|left|200px]]<br /><applet load="2nyt" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2nyt" size=" | + | |
caption="2nyt, resolution 2.5Å" /> | caption="2nyt, resolution 2.5Å" /> | ||
'''The APOBEC2 Crystal Structure and Functional Implications for AID'''<br /> | '''The APOBEC2 Crystal Structure and Functional Implications for AID'''<br /> | ||
| Line 8: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2NYT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2NYT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NYT OCA]. |
==Reference== | ==Reference== | ||
| Line 24: | Line 23: | ||
[[Category: zinc-ion binding]] | [[Category: zinc-ion binding]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:03:43 2008'' |
Revision as of 13:03, 23 January 2008
|
The APOBEC2 Crystal Structure and Functional Implications for AID
Overview
APOBEC-2 (APO2) belongs to the family of apolipoprotein B messenger, RNA-editing enzyme catalytic (APOBEC) polypeptides, which deaminates mRNA, and single-stranded DNA. Different APOBEC members use the same deamination, activity to achieve diverse human biological functions. Deamination by an, APOBEC protein called activation-induced cytidine deaminase (AID) is, critical for generating high-affinity antibodies, and deamination by, APOBEC-3 proteins can inhibit retrotransposons and the replication of, retroviruses such as human immunodeficiency virus and hepatitis B virus., Here we report the crystal structure of APO2. APO2 forms a rod-shaped, tetramer that differs markedly from the square-shaped tetramer of the free, nucleotide cytidine deaminase, with which APOBEC proteins share, considerable sequence homology. In APO2, two long alpha-helices of a, monomer structure prevent the formation of a square-shaped tetramer and, facilitate formation of the rod-shaped tetramer via head-to-head, interactions of two APO2 dimers. Extensive sequence homology among APOBEC, family members allows us to test APO2 structure-based predictions using, AID. We show that AID deamination activity is impaired by mutations, predicted to interfere with oligomerization and substrate access. The, structure suggests how mutations in patients with hyper-IgM-2 syndrome, inactivate AID, resulting in defective antibody maturation.
About this Structure
2NYT is a Single protein structure of sequence from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
Reference
The APOBEC-2 crystal structure and functional implications for the deaminase AID., Prochnow C, Bransteitter R, Klein MG, Goodman MF, Chen XS, Nature. 2007 Jan 25;445(7126):447-51. Epub 2006 Dec 24. PMID:17187054
Page seeded by OCA on Wed Jan 23 15:03:43 2008
