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	== '''Inorganic Pyrophosphatase''' ==		== '''Inorganic Pyrophosphatase''' ==
-	E-PPase, a homohexameric protein <ref name = "wong"> PMID: 5498422 </ref>, contains 175 amino-acid residues in each subunit <ref name = "lahti"> PMID: 2848015 </ref>.	+
	__TOC__		__TOC__
	==Structure==		==Structure==
-		+	E-PPase, a homohexameric protein <ref name = "wong"> PMID: 5498422 </ref>, contains 175 amino-acid residues in each subunit <ref name = "lahti"> PMID: 2848015 </ref>.
	==Function==		==Function==

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Revision as of 22:37, 31 March 2011

spinqualitylabelsall models PDB ID 1faj Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image
1faj, resolution 2.15Å ()
Activity:	Inorganic diphosphatase, with EC number 3.6.1.1
Structural annotation: Resources: CATH : 1Faj000 InterPro : Ipr008162 Pfam : PF00719 SCOP : d1faj__ UniProt : P0A7A9
Functional annotation: Cellular component: cytoplasm membrane fraction Biological process: phosphate metabolic process Molecular function: hydrolase activity magnesium ion binding metal ion binding inorganic diphosphatase activity
Evolutionary conservation: Toggle Conservation Colors: Rows = identical sequences: Further Information: Caveats, Explanation, Complete Results at ConSurfDB
Resources:	FirstGlance, OCA, PDBsum, RCSB
Coordinates:	save as pdb, mmCIF, xml

Soluble inorganic pyrophosphatase is a ubiquitous enzyme that plays an important role in energy metabolism ^[1]. Energy metabolism is made possible by soluble inorganic pyrophosphatases (PPases) by their hydrolyzing inorganic phosphates into two molecules of orthophosphate ^[2]. PPases may have had an important role in evolution by aiding in accurate DNA copying during chromosome duplication ^[1]. Escherichia coli (E-coli bacteria) and Saccharomyces cerevisiae (S. cerevisiae yeast) PPases, E-PPase and Y-PPase respectively, are the two best studied PPases ^[1].

Inorganic Pyrophosphatase

Contents 1 Inorganic Pyrophosphatase 2 Structure 3 Function 4 References

Structure

E-PPase, a homohexameric protein ^[3], contains 175 amino-acid residues in each subunit ^[4].

Function

References

1. ↑ ^{1.0 1.1 1.2} Kankare J, Neal GS, Salminen T, Glumoff T, Glumhoff T [corrected to Glumoff T, Cooperman BS, Lahti R, Goldman A. The structure of E.coli soluble inorganic pyrophosphatase at 2.7 A resolution. Protein Eng. 1994 Jul;7(7):823-30. PMID:7971944
2. ↑ Samygina VR, Popov AN, Rodina EV, Vorobyeva NN, Lamzin VS, Polyakov KM, Kurilova SA, Nazarova TI, Avaeva SM. The structures of Escherichia coli inorganic pyrophosphatase complexed with Ca(2+) or CaPP(i) at atomic resolution and their mechanistic implications. J Mol Biol. 2001 Nov 30;314(3):633-45. PMID:11846572 doi:10.1006/jmbi.2001.5149
3. ↑ Wong SC, Burton PM, Josse J. Constitutive inorganic pyrophosphatase of Escherichia coli. V. Reconstitution of native enzyme particles from subunit polypeptide chains. J Biol Chem. 1970 Sep 10;245(17):4353-7. PMID:5498422
4. ↑ Lahti R, Pitkaranta T, Valve E, Ilta I, Kukko-Kalske E, Heinonen J. Cloning and characterization of the gene encoding inorganic pyrophosphatase of Escherichia coli K-12. J Bacteriol. 1988 Dec;170(12):5901-7. PMID:2848015