This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1e9g
From Proteopedia
(New page: 200px<br /> <applet load="1e9g" size="450" color="white" frame="true" align="right" spinBox="true" caption="1e9g, resolution 1.15Å" /> '''STRUCTURE OF INORGA...) |
|||
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1E9G is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]] with MN and PO4 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.1 3.6.1.1]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1E9G OCA]]. | + | 1E9G is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]] with MN and PO4 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Inorganic_diphosphatase Inorganic diphosphatase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.1 3.6.1.1]]. Structure known Active Sites: ASA and ASB. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1E9G OCA]]. |
==Reference== | ==Reference== | ||
Toward a quantum-mechanical description of metal-assisted phosphoryl transfer in pyrophosphatase., Heikinheimo P, Tuominen V, Ahonen AK, Teplyakov A, Cooperman BS, Baykov AA, Lahti R, Goldman A, Proc Natl Acad Sci U S A. 2001 Mar 13;98(6):3121-6. Epub 2001 Mar 6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11248042 11248042] | Toward a quantum-mechanical description of metal-assisted phosphoryl transfer in pyrophosphatase., Heikinheimo P, Tuominen V, Ahonen AK, Teplyakov A, Cooperman BS, Baykov AA, Lahti R, Goldman A, Proc Natl Acad Sci U S A. 2001 Mar 13;98(6):3121-6. Epub 2001 Mar 6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11248042 11248042] | ||
| + | [[Category: Inorganic diphosphatase]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 28: | Line 29: | ||
[[Category: pyrophosphate phosphohydrolase]] | [[Category: pyrophosphate phosphohydrolase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:50:43 2007'' |
Revision as of 09:46, 30 October 2007
|
STRUCTURE OF INORGANIC PYROPHOSPHATASE
Overview
The wealth of kinetic and structural information makes inorganic, pyrophosphatases (PPases) a good model system to study the details of, enzymatic phosphoryl transfer. The enzyme accelerates metal-complexed, phosphoryl transfer 10(10)-fold: but how? Our structures of the yeast, PPase product complex at 1.15 A and fluoride-inhibited complex at 1.9 A, visualize the active site in three different states: substrate-bound, immediate product bound, and relaxed product bound. These span the steps, around chemical catalysis and provide strong evidence that a water, molecule (O(nu)) directly attacks PPi with a pK(a) vastly lowered by, coordination to two metal ions and D117. They also suggest that a, low-barrier hydrogen bond (LBHB) forms between D117 and O(nu), in part, because of steric crowding ... [(full description)]
About this Structure
1E9G is a [Single protein] structure of sequence from [Saccharomyces cerevisiae] with MN and PO4 as [ligands]. Active as [Inorganic diphosphatase], with EC number [3.6.1.1]. Structure known Active Sites: ASA and ASB. Full crystallographic information is available from [OCA].
Reference
Toward a quantum-mechanical description of metal-assisted phosphoryl transfer in pyrophosphatase., Heikinheimo P, Tuominen V, Ahonen AK, Teplyakov A, Cooperman BS, Baykov AA, Lahti R, Goldman A, Proc Natl Acad Sci U S A. 2001 Mar 13;98(6):3121-6. Epub 2001 Mar 6. PMID:11248042
Page seeded by OCA on Tue Oct 30 11:50:43 2007
