1aj8
From Proteopedia
(New page: 200px<br /> <applet load="1aj8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1aj8, resolution 1.9Å" /> '''CITRATE SYNTHASE FRO...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1AJ8 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]] with COA and CIT as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.3.7 4.1.3.7]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AJ8 OCA]]. | + | 1AJ8 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]] with COA and CIT as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Transferred_entry:_2.3.3.1 Transferred entry: 2.3.3.1]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.3.7 4.1.3.7]]. Structure known Active Sites: ACA and ACB. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AJ8 OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: Pyrococcus furiosus]] | [[Category: Pyrococcus furiosus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| + | [[Category: Transferred entry: 2.3.3.1]] | ||
[[Category: Danson, M.J.]] | [[Category: Danson, M.J.]] | ||
[[Category: Ferguson, J.M.C.]] | [[Category: Ferguson, J.M.C.]] | ||
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[[Category: lyase]] | [[Category: lyase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:51:10 2007'' |
Revision as of 09:46, 30 October 2007
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CITRATE SYNTHASE FROM PYROCOCCUS FURIOSUS
Overview
The crystal structure of the closed form of citrate synthase, with citrate, and CoA bound, from the hyperthermophilic Archaeon Pyrococcus furiosus has, been determined to 1.9 A. This has allowed direct structural comparisons, between the same enzyme from organisms growing optimally at 37 degrees C, (pig), 55 degrees C (Thermoplasma acidophilum) and now 100 degrees C, (Pyrococcus furiosus). The three enzymes are homodimers and share a, similar overall fold, with the dimer interface comprising primarily an, eight alpha-helical sandwich of four antiparallel pairs of helices. The, active sites show similar modes of substrate binding; moreover, the, structural equivalence of the amino acid residues implicated in catalysis, implies that the mechanism proceeds via the same acid-base catalytic, ... [(full description)]
About this Structure
1AJ8 is a [Single protein] structure of sequence from [Pyrococcus furiosus] with COA and CIT as [ligands]. Active as [Transferred entry: 2.3.3.1], with EC number [4.1.3.7]. Structure known Active Sites: ACA and ACB. Full crystallographic information is available from [OCA].
Reference
The crystal structure of citrate synthase from the hyperthermophilic archaeon pyrococcus furiosus at 1.9 A resolution,., Russell RJ, Ferguson JM, Hough DW, Danson MJ, Taylor GL, Biochemistry. 1997 Aug 19;36(33):9983-94. PMID:9254593
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