2o6l
From Proteopedia
(New page: 200px<br /> <applet load="2o6l" size="450" color="white" frame="true" align="right" spinBox="true" caption="2o6l, resolution 1.80Å" /> '''Crystal Structure o...) |
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'''Crystal Structure of the UDP-Glucuronic Acid Binding Domain of the Human Drug Metabolizing UDP-Glucuronosyltransferase 2B7'''<br /> | '''Crystal Structure of the UDP-Glucuronic Acid Binding Domain of the Human Drug Metabolizing UDP-Glucuronosyltransferase 2B7'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2O6L is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/Glucuronosyltransferase Glucuronosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.17 2.4.1.17] Full crystallographic information is available from [http:// | + | 2O6L is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/Glucuronosyltransferase Glucuronosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.17 2.4.1.17] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O6L OCA]. |
==Reference== | ==Reference== | ||
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[[Category: ugt]] | [[Category: ugt]] | ||
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Revision as of 13:09, 23 January 2008
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Crystal Structure of the UDP-Glucuronic Acid Binding Domain of the Human Drug Metabolizing UDP-Glucuronosyltransferase 2B7
Overview
Human UDP-glucuronosyltransferases (UGT) are the dominant phase II, conjugative drug metabolism enzymes that also play a central role in, processing a range of endobiotic compounds. UGTs catalyze the covalent, addition of glucuronic acid sugar moieties to a host of therapeutics and, environmental toxins, as well as to a variety of endogenous steroids and, other signaling molecules. We report the 1.8-A resolution apo crystal, structure of the UDP-glucuronic acid binding domain of human UGT isoform, 2B7 (UGT2B7), which catalyzes the conjugative elimination of opioid, antiviral, and anticancer drugs. This is the first crystal structure of, any region of a mammalian UGT drug metabolism enzyme. Designated UGT2B7, mutants at residues predicted to interact with the UDP-glucuronic acid, cofactor exhibited significantly impaired catalytic activity, with maximum, effects observed for amino acids closest to the glucuronic acid sugar, transferred to the acceptor molecule. Homology modeling of UGT2B7 with, related plant flavonoid glucosyltransferases indicates human UGTs share a, common catalytic mechanism. Point mutations at predicted catalytic, residues in UGT2B7 abrogated activity, strongly suggesting human UGTs also, utilize a serine hydrolase-like catalytic mechanism to facilitate, glucuronic acid transfer.
About this Structure
2O6L is a Single protein structure of sequence from Homo sapiens. Active as Glucuronosyltransferase, with EC number 2.4.1.17 Full crystallographic information is available from OCA.
Reference
Crystal Structure of the Cofactor-Binding Domain of the Human Phase II Drug-Metabolism Enzyme UDP-Glucuronosyltransferase 2B7., Miley MJ, Zielinska AK, Keenan JE, Bratton SM, Radominska-Pandya A, Redinbo MR, J Mol Biol. 2007 Mar 30;. PMID:17442341
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