2han
From Proteopedia
(New page: 200px<br /><applet load="2han" size="450" color="white" frame="true" align="right" spinBox="true" caption="2han, resolution 1.95Å" /> '''Structural basis of ...) |
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| - | [[Image:2han.gif|left|200px]]<br /><applet load="2han" size=" | + | [[Image:2han.gif|left|200px]]<br /><applet load="2han" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2han, resolution 1.95Å" /> | caption="2han, resolution 1.95Å" /> | ||
'''Structural basis of heterodimeric ecdysteroid receptor interaction with natural response element hsp27 gene promoter'''<br /> | '''Structural basis of heterodimeric ecdysteroid receptor interaction with natural response element hsp27 gene promoter'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2HAN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2HAN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HAN OCA]. |
==Reference== | ==Reference== | ||
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[[Category: zinc finger]] | [[Category: zinc finger]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:11:22 2008'' |
Revision as of 13:11, 23 January 2008
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Structural basis of heterodimeric ecdysteroid receptor interaction with natural response element hsp27 gene promoter
Overview
The heterodimer of the ecdysone receptor (EcR) and ultraspiracle (Usp), members of the nuclear receptors superfamily, is considered as the, functional receptor for ecdysteroids initiating molting and metamorphosis, in insects. Here we report the 1.95 A structure of the complex formed by, the DNA-binding domains (DBDs) the EcR and the Usp, bound to the natural, pseudopalindromic response element. Comparison of the structure with that, obtained previously, using an idealized response element, shows how the, EcRDBD, which has been previously reported to possess extraordinary, flexibility, accommodates DNA-induced structural changes. Part of the, C-terminal extension (CTE) of the EcRDBD folds into an alpha-helix whose, location in the minor groove does not match any of the locations, previously observed for nuclear receptors. Mutational analyses suggest, that the alpha-helix is a component of EcR-box, a novel element, indispensable for DNA-binding and located within the nuclear receptor CTE., This element seems to be a general feature of all known EcRs.
About this Structure
2HAN is a Protein complex structure of sequences from Drosophila melanogaster with as ligand. Full crystallographic information is available from OCA.
Reference
Novel DNA-binding element within the C-terminal extension of the nuclear receptor DNA-binding domain., Jakob M, Kolodziejczyk R, Orlowski M, Krzywda S, Kowalska A, Dutko-Gwozdz J, Gwozdz T, Kochman M, Jaskolski M, Ozyhar A, Nucleic Acids Res. 2007;35(8):2705-18. Epub 2007 Apr 10. PMID:17426125
Page seeded by OCA on Wed Jan 23 15:11:22 2008
Categories: Drosophila melanogaster | Protein complex | Dutko-Gwozdz, J. | Gwozdz, T. | Jakob, M. | Jaskolski, M. | Kochman, M. | Kolodziejczyk, R. | Kowalska, A. | Krzywda, S. | Orlowski, M. | Ozyhar, A. | ZN | Dna-binding | Nuclear protein | Nuclear receptor | Transcription factor | Transcription regulation | Zinc finger
