2olc
From Proteopedia
(New page: 200px<br /><applet load="2olc" size="450" color="white" frame="true" align="right" spinBox="true" caption="2olc, resolution 2.000Å" /> '''Crystal structure o...) |
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| - | [[Image:2olc.jpg|left|200px]]<br /><applet load="2olc" size=" | + | [[Image:2olc.jpg|left|200px]]<br /><applet load="2olc" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2olc, resolution 2.000Å" /> | caption="2olc, resolution 2.000Å" /> | ||
'''Crystal structure of 5-methylthioribose kinase in complex with ADP-2Ho'''<br /> | '''Crystal structure of 5-methylthioribose kinase in complex with ADP-2Ho'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2OLC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with HO, CPS and ADP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/S-methyl-5-thioribose_kinase S-methyl-5-thioribose kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.100 2.7.1.100] Full crystallographic information is available from [http:// | + | 2OLC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with <scene name='pdbligand=HO:'>HO</scene>, <scene name='pdbligand=CPS:'>CPS</scene> and <scene name='pdbligand=ADP:'>ADP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/S-methyl-5-thioribose_kinase S-methyl-5-thioribose kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.100 2.7.1.100] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OLC OCA]. |
==Reference== | ==Reference== | ||
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[[Category: kinase adp-2ho complex]] | [[Category: kinase adp-2ho complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:12:29 2008'' |
Revision as of 13:12, 23 January 2008
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Crystal structure of 5-methylthioribose kinase in complex with ADP-2Ho
Overview
Trivalent holmium ions were shown to isomorphously replace magnesium ions, to form an ADP-2Ho complex in the nucleotide-binding domain of Bacillus, subtilis 5-methylthioribose (MTR) kinase. This nucleotide-holmium complex, provided sufficient phasing power to allow SAD and SIRAS phasing of this, previously unknown structure using the L(III) absorption edge of holmium., The structure of ADP-2Ho reveals that the two Ho ions are approximately 4, A apart and are likely to share their ligands: the phosphoryl O atoms of, ADP and a water molecule. The structure determination of MTR kinase using, data collected using Cu Kalpha X-radiation was also attempted. Although, the heavy-atom substructure determination was successful, interpretation, of the map was more challenging. The isomorphous substitution of holmium, for magnesium in the MTR kinase-nucleotide complex suggests that this, could be a useful phasing tool for other metal-dependent, nucleotide-containing proteins.
About this Structure
2OLC is a Single protein structure of sequence from Bacillus subtilis with , and as ligands. Active as S-methyl-5-thioribose kinase, with EC number 2.7.1.100 Full crystallographic information is available from OCA.
Reference
ADP-2Ho as a phasing tool for nucleotide-containing proteins., Ku SY, Smith GD, Howell PL, Acta Crystallogr D Biol Crystallogr. 2007 Apr;63(Pt 4):493-9. Epub 2007, Mar 16. PMID:17372354
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