2e9x
From Proteopedia
(New page: 200px<br /> <applet load="2e9x" size="450" color="white" frame="true" align="right" spinBox="true" caption="2e9x, resolution 2.30Å" /> '''The crystal structu...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:2e9x. | + | [[Image:2e9x.jpg|left|200px]]<br /><applet load="2e9x" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2e9x" size=" | + | |
caption="2e9x, resolution 2.30Å" /> | caption="2e9x, resolution 2.30Å" /> | ||
'''The crystal structure of human GINS core complex'''<br /> | '''The crystal structure of human GINS core complex'''<br /> | ||
| Line 6: | Line 5: | ||
==Overview== | ==Overview== | ||
The eukaryotic GINS complex is essential for the establishment of DNA, replication forks and replisome progression. We report the crystal, structure of the human GINS complex. The heterotetrameric complex adopts a, pseudo symmetrical layered structure comprising two heterodimers, creating, four subunit-subunit interfaces. The subunit structures of the, heterodimers consist of two alternating domains. The C-terminal domains of, the Sld5 and Psf1 subunits are connected by linker regions to the core, complex, and the C-terminal domain of Sld5 is important for core complex, assembly. In contrast, the C-terminal domain of Psf1 does not contribute, to the stability of the complex but is crucial for chromatin binding and, replication activity. These data suggest that the core complex ensures a, stable platform for the C-terminal domain of Psf1 to act as a key, interaction interface for other proteins in the replication-initiation, process. | The eukaryotic GINS complex is essential for the establishment of DNA, replication forks and replisome progression. We report the crystal, structure of the human GINS complex. The heterotetrameric complex adopts a, pseudo symmetrical layered structure comprising two heterodimers, creating, four subunit-subunit interfaces. The subunit structures of the, heterodimers consist of two alternating domains. The C-terminal domains of, the Sld5 and Psf1 subunits are connected by linker regions to the core, complex, and the C-terminal domain of Sld5 is important for core complex, assembly. In contrast, the C-terminal domain of Psf1 does not contribute, to the stability of the complex but is crucial for chromatin binding and, replication activity. These data suggest that the core complex ensures a, stable platform for the C-terminal domain of Psf1 to act as a key, interaction interface for other proteins in the replication-initiation, process. | ||
| - | |||
| - | ==Disease== | ||
| - | Known diseases associated with this structure: Bare lymphocyte syndrome, type I OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=170260 170260]], Bare lymphocyte syndrome, type I, due to TAP2 deficiency OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=170261 170261]], Wegener-like granulomatosis OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=170261 170261]] | ||
==About this Structure== | ==About this Structure== | ||
| - | 2E9X is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2E9X is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E9X OCA]. |
==Reference== | ==Reference== | ||
| Line 23: | Line 19: | ||
[[Category: gins complex]] | [[Category: gins complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:14:39 2008'' |
Revision as of 13:14, 23 January 2008
|
The crystal structure of human GINS core complex
Overview
The eukaryotic GINS complex is essential for the establishment of DNA, replication forks and replisome progression. We report the crystal, structure of the human GINS complex. The heterotetrameric complex adopts a, pseudo symmetrical layered structure comprising two heterodimers, creating, four subunit-subunit interfaces. The subunit structures of the, heterodimers consist of two alternating domains. The C-terminal domains of, the Sld5 and Psf1 subunits are connected by linker regions to the core, complex, and the C-terminal domain of Sld5 is important for core complex, assembly. In contrast, the C-terminal domain of Psf1 does not contribute, to the stability of the complex but is crucial for chromatin binding and, replication activity. These data suggest that the core complex ensures a, stable platform for the C-terminal domain of Psf1 to act as a key, interaction interface for other proteins in the replication-initiation, process.
About this Structure
2E9X is a Protein complex structure of sequences from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
Reference
Structure of the human GINS complex and its assembly and functional interface in replication initiation., Kamada K, Kubota Y, Arata T, Shindo Y, Hanaoka F, Nat Struct Mol Biol. 2007 May;14(5):388-96. Epub 2007 Apr 8. PMID:17417653
Page seeded by OCA on Wed Jan 23 15:14:39 2008
