2e5l
From Proteopedia
(New page: 200px<br /><applet load="2e5l" size="450" color="white" frame="true" align="right" spinBox="true" caption="2e5l, resolution 3.300Å" /> '''A snapshot of the 3...) |
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| - | [[Image:2e5l.gif|left|200px]]<br /><applet load="2e5l" size=" | + | [[Image:2e5l.gif|left|200px]]<br /><applet load="2e5l" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2e5l, resolution 3.300Å" /> | caption="2e5l, resolution 3.300Å" /> | ||
'''A snapshot of the 30S ribosomal subunit capturing mRNA via the Shine- Dalgarno interaction'''<br /> | '''A snapshot of the 30S ribosomal subunit capturing mRNA via the Shine- Dalgarno interaction'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2E5L is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2E5L is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E5L OCA]. |
==Reference== | ==Reference== | ||
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[[Category: translation initiation]] | [[Category: translation initiation]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:15:44 2008'' |
Revision as of 13:15, 23 January 2008
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A snapshot of the 30S ribosomal subunit capturing mRNA via the Shine- Dalgarno interaction
Overview
In the initiation phase of bacterial translation, the 30S ribosomal, subunit captures mRNA in preparation for binding with initiator tRNA. The, purine-rich Shine-Dalgarno (SD) sequence, in the 5' untranslated region of, the mRNA, anchors the 30S subunit near the start codon, via base pairing, with an anti-SD (aSD) sequence at the 3' terminus of 16S rRNA. Here, we, present the 3.3 A crystal structure of the Thermus thermophilus 30S, subunit bound with an mRNA mimic. The duplex formed by the SD and aSD, sequences is snugly docked in a "chamber" between the head and platform, domains, demonstrating how the 30S subunit captures and stabilizes the, otherwise labile SD helix. This location of the SD helix is suitable for, the placement of the start codon AUG in the immediate vicinity of the mRNA, channel, in agreement with reported crosslinks between the second position, of the start codon and G1530 of 16S rRNA.
About this Structure
2E5L is a Protein complex structure of sequences from Thermus thermophilus with as ligand. Full crystallographic information is available from OCA.
Reference
A snapshot of the 30S ribosomal subunit capturing mRNA via the Shine-Dalgarno interaction., Kaminishi T, Wilson DN, Takemoto C, Harms JM, Kawazoe M, Schluenzen F, Hanawa-Suetsugu K, Shirouzu M, Fucini P, Yokoyama S, Structure. 2007 Mar;15(3):289-97. PMID:17355865
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Categories: Protein complex | Thermus thermophilus | Fucini, P. | Hanawa-Suetsugu, K. | Harms, J.M. | Kaminishi, T. | Kawazoe, M. | RSGI, RIKEN.Structural.Genomics/Proteomics.Initiative. | Schluenzen, F. | Shirouzu, M. | Takemoto, C. | Wilson, D.N. | Yokoyama, S. | ZN | 30s ribosomal subunit | 5' untranslated region | Mrna capture | National project on protein structural and functional analyses | Nppsfa | Riken structural genomics/proteomics initiative | Rsgi | Shine-dalgarno interaction | Structural genomics | Translation initiation
