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| - | [[Image:2gzt.gif|left|200px]]<br /><applet load="2gzt" size="450" color="white" frame="true" align="right" spinBox="true" | + | [[Image:2gzt.gif|left|200px]]<br /><applet load="2gzt" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | caption="2gzt, resolution 1.70Å" /> | + | caption="2gzt" /> |
| - | '''Crystal structure of the HutP antitermination complex bound to the HUT mRNA'''<br /> | + | ''''''<br /> |
| - | | + | |
| - | ==Overview==
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| - | HutP is an L-histidine-activated RNA binding protein that regulates the, expression of the histidine utilization (hut) operon in Bacillus subtilis, by binding to cis-acting regulatory sequences on the hut mRNA. The crystal, structure of HutP complexed with an L-histidine analog showed a novel, fold; there are four antiparallel beta strands in the central region of, each monomer, with two alpha helices each on the front and back. Two HutP, monomers form a dimer, and three dimers are arranged in crystallographic, 3-fold symmetry to form a hexamer. A histidine analog was located in, between the two monomers of HutP, with the imidazole group of L-histidine, hydrogen bonded to Glu81. An activation mechanism is proposed based on the, identification of key residues of HutP. The HutP binding region in hut, mRNA was defined: it consists of three UAG trinucleotide motifs separated, by four spacer nucleotides. Residues of HutP potentially important for RNA, binding were identified.
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| | ==About this Structure== | | ==About this Structure== |
| - | 2GZT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with MG and HIS as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2GZT OCA].
| + | is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. |
| - | | + | [[Category: Protein complex]] |
| - | ==Reference==
| + | |
| - | Crystal structure of activated HutP; an RNA binding protein that regulates transcription of the hut operon in Bacillus subtilis., Kumarevel T, Fujimoto Z, Karthe P, Oda M, Mizuno H, Kumar PK, Structure. 2004 Jul;12(7):1269-80. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15242603 15242603]
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| - | [[Category: Bacillus subtilis]] | + | |
| - | [[Category: Single protein]]
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| - | [[Category: Balasundaresan, D.]]
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| - | [[Category: Jeyakanthan, J.]]
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| - | [[Category: Kumar, P.K.R.]]
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| - | [[Category: Kumarevel, T.S.]]
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| - | [[Category: RSGI, RIKEN.Structural.Genomics/Proteomics.Initiative.]]
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| - | [[Category: Shinkai, A.]]
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| - | [[Category: Yokoyama, S.]]
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| - | [[Category: HIS]]
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| - | [[Category: MG]]
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| - | [[Category: antitermination]]
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| - | [[Category: hutp]]
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| - | [[Category: hutp-rna complex]]
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| - | [[Category: national project on protein structural and functional analyses]]
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| - | [[Category: nppsfa]]
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| - | [[Category: riken structural genomics/proteomics initiative]]
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| - | [[Category: rna binding]]
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| - | [[Category: rsgi]]
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| - | [[Category: structural genomics]]
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| - | [[Category: transcription regulation]]
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| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 11:28:26 2007'' | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:15:53 2008'' |
