2gzw

From Proteopedia

Revision as of 13:15, 23 January 2008

Crystal structure of the E.coli CRP-cAMP complex

Overview

The structure of a dimer of the Escherichia coli catabolite gene activator, protein has been refined at 2.5 A resolution to a crystallographic, R-factor of 20.7% starting with coordinates fitted to the map at 2.9 A, resolution. The two subunits are in different conformations and each, contains one bound molecule of the allosteric activator, cyclic AMP. The, amino-terminal domain is linked to the smaller carboxy-terminal domain by, a nine-residue hinge region that exists in different conformations in the, two subunits, giving rise to approximately a 30 degree rotation between, the positions of the small domains relative to the larger domains. The, amino-terminal domain contains an antiparallel beta-roll structure in, which the interstrand hydrogen bonding is well-determined. The beta-roll, can be described as a long antiparallel beta-ribbon that folds into a, right-handed supercoil and forms part of the cyclic AMP binding site. Each, cyclic AMP molecule is in an anti conformation and has ionic and hydrogen, bond interactions with both subunits.

About this Structure

2GZW is a Single protein structure of sequence from Escherichia coli with as ligand. Full crystallographic information is available from OCA.

Reference

Structure of a complex of catabolite gene activator protein and cyclic AMP refined at 2.5 A resolution., Weber IT, Steitz TA, J Mol Biol. 1987 Nov 20;198(2):311-26. PMID:2828639

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