2owo
From Proteopedia
(New page: 200px<br /><applet load="2owo" size="450" color="white" frame="true" align="right" spinBox="true" caption="2owo, resolution 2.300Å" /> '''Last Stop on the Ro...) |
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| - | [[Image:2owo.gif|left|200px]]<br /><applet load="2owo" size=" | + | [[Image:2owo.gif|left|200px]]<br /><applet load="2owo" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2owo, resolution 2.300Å" /> | caption="2owo, resolution 2.300Å" /> | ||
'''Last Stop on the Road to Repair: Structure of E.coli DNA Ligase Bound to Nicked DNA-Adenylate'''<br /> | '''Last Stop on the Road to Repair: Structure of E.coli DNA Ligase Bound to Nicked DNA-Adenylate'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2OWO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ZN, SO4 and AMP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA_ligase_(NAD(+)) DNA ligase (NAD(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.5.1.2 6.5.1.2] Full crystallographic information is available from [http:// | + | 2OWO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=AMP:'>AMP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA_ligase_(NAD(+)) DNA ligase (NAD(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.5.1.2 6.5.1.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OWO OCA]. |
==Reference== | ==Reference== | ||
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[[Category: protein/dna complex]] | [[Category: protein/dna complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:17:07 2008'' |
Revision as of 13:17, 23 January 2008
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Last Stop on the Road to Repair: Structure of E.coli DNA Ligase Bound to Nicked DNA-Adenylate
Overview
NAD(+)-dependent DNA ligases (LigA) are ubiquitous in bacteria and, essential for growth. Their distinctive substrate specificity and domain, organization vis-a-vis human ATP-dependent ligases make them outstanding, targets for anti-infective drug discovery. We report here the 2.3 A, crystal structure of Escherichia coli LigA bound to an adenylylated nick, which captures LigA in a state poised for strand closure and reveals the, basis for nick recognition. LigA envelops the DNA within a protein clamp., Large protein domain movements and remodeling of the active site, orchestrate progression through the three chemical steps of the ligation, reaction. The structure inspires a strategy for inhibitor design.
About this Structure
2OWO is a Single protein structure of sequence from Escherichia coli with , and as ligands. Active as DNA ligase (NAD(+)), with EC number 6.5.1.2 Full crystallographic information is available from OCA.
Reference
Last Stop on the Road to Repair: Structure of E. coli DNA Ligase Bound to Nicked DNA-Adenylate., Nandakumar J, Nair PA, Shuman S, Mol Cell. 2007 Apr 27;26(2):257-71. PMID:17466627
Page seeded by OCA on Wed Jan 23 15:17:07 2008
Categories: DNA ligase (NAD(+)) | Escherichia coli | Single protein | Nair, P.A. | Nandakumar, J. | Shuman, S. | AMP | SO4 | ZN | Dna | Ligase | Protein/dna complex
