Journal:JBIC:10
From Proteopedia
(Difference between revisions)
| Line 4: | Line 4: | ||
<hr/> | <hr/> | ||
<b>Molecular Tour</b><br> | <b>Molecular Tour</b><br> | ||
| - | Structures of the all cysteinyl coordinated D14C variant of ferredoxin from the hyperthermophilic archaeon ''Pyrococcus furiosus'' have been determined for the [4Fe-4S] - and [3Fe-4S] forms. | + | Structures of the all cysteinyl coordinated D14C variant of ferredoxin from the hyperthermophilic archaeon ''Pyrococcus furiosus'' have been determined for the [4Fe-4S] - and [3Fe-4S] forms. The [4Fe-4S] form diffracted to 1.7 Å and two different types of molecules were found in the crystal. They have different crystal packing and intramolecular disulfide bond conformation. The crystal packing reveals a beta-sheet interaction between A molecules in adjacent asymmetric units, while B molecules are packed as monomers in a less rigid position next to the A-A extended beta-sheet dimers. The intramolecular disulfide bond in the |
| + | A molecules is in a double conformation while the intramolecular disulfide bond in the B molecules is in a single conformation. | ||
| - | The [ | + | Two forms of D14C [3Fe-4S] ''Pyrococcus furiosus'' ferredoxin are obtained when purified at pH 8.0: a monomer and a dimer connected by an intermolecular disulfide bond. When purified at pH 5.8, only the monomer is obtained. The [3Fe-4S] form diffracted to 2.8 Å resolution and showed only the monomeric form, which resembles molecule A of D14C [4Fe-4S] ''Pyrococcus furiosus'' ferredoxin. |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | A- | + | |
| - | + | ||
| - | + | ||
| - | Two forms of D14C [3Fe-4S] ''Pyrococcus furiosus'' ferredoxin are obtained | ||
| - | when purified at pH 8.0: a monomer and a dimer connected by an intermolecular | ||
| - | disulfide bond. When purified at pH 5.8, only the monomer is obtained. | ||
| - | The [3Fe-4S] form diffracted to 2.8 Å resolution and showed only the | ||
| - | monomeric form, which resembles molecule A of D14C [4Fe-4S] | ||
| - | ''Pyrococcus furiosus'' ferredoxin. | ||
| - | <scene name='Journal:JBIC:10/Cv/1'>TextToBeDisplayed</scene> <scene name='Journal:JBIC:10/Cv/2'>TextToBeDisplayed</scene> | ||
| - | <scene name='Journal:JBIC:10/Cv/3'>TextToBeDisplayed</scene> | ||
</StructureSection> | </StructureSection> | ||
<references/> | <references/> | ||
__NOEDITSECTION__ | __NOEDITSECTION__ | ||
Revision as of 07:31, 7 April 2011
| |||||||||||
- ↑ DOI
This page complements a publication in scientific journals and is one of the Proteopedia's Interactive 3D Complement pages. For aditional details please see I3DC.
