2ote
From Proteopedia
(New page: 200px<br /><applet load="2ote" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ote, resolution 1.47Å" /> '''Crystal structure of...) |
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| - | [[Image:2ote.jpg|left|200px]]<br /><applet load="2ote" size=" | + | [[Image:2ote.jpg|left|200px]]<br /><applet load="2ote" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2ote, resolution 1.47Å" /> | caption="2ote, resolution 1.47Å" /> | ||
'''Crystal structure of a monomeric cyan fluorescent protein in the photobleached state'''<br /> | '''Crystal structure of a monomeric cyan fluorescent protein in the photobleached state'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2OTE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Clavularia_sp. Clavularia sp.] with ACT as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2OTE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Clavularia_sp. Clavularia sp.] with <scene name='pdbligand=ACT:'>ACT</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OTE OCA]. |
==Reference== | ==Reference== | ||
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[[Category: fluorescent protein]] | [[Category: fluorescent protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:19:38 2008'' |
Revision as of 13:19, 23 January 2008
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Crystal structure of a monomeric cyan fluorescent protein in the photobleached state
Overview
Fluorescent protein (FP) variants that can be reversibly converted between, fluorescent and nonfluorescent states have proven to be a catalyst for, innovation in the field of fluorescence microscopy. However, the, structural basis of the process remains poorly understood. High-resolution, structures of a FP derived from Clavularia in both the fluorescent and the, light-induced nonfluorescent states reveal that the rapid and complete, loss of fluorescence observed upon illumination with 450-nm light results, from cis-trans isomerization of the chromophore. The photoinduced change, in configuration from the well ordered cis isomer to the highly nonplanar, and disordered trans isomer is accompanied by a dramatic rearrangement of, internal side chains. Taken together, the structures provide an, explanation for the loss of fluorescence upon illumination, the slow, light-independent recovery, and the rapid light-induced recovery of, fluorescence. The fundamental mechanism appears to be common to all of the, photoactivatable and reversibly photoswitchable FPs reported to date.
About this Structure
2OTE is a Single protein structure of sequence from Clavularia sp. with as ligand. Full crystallographic information is available from OCA.
Reference
Structural basis for reversible photobleaching of a green fluorescent protein homologue., Henderson JN, Ai HW, Campbell RE, Remington SJ, Proc Natl Acad Sci U S A. 2007 Apr 17;104(16):6672-7. Epub 2007 Apr 9. PMID:17420458
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