2p32
From Proteopedia
(New page: 200px<br /><applet load="2p32" size="450" color="white" frame="true" align="right" spinBox="true" caption="2p32, resolution 3.200Å" /> '''Crystal structure o...) |
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| - | [[Image:2p32.gif|left|200px]]<br /><applet load="2p32" size=" | + | [[Image:2p32.gif|left|200px]]<br /><applet load="2p32" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2p32, resolution 3.200Å" /> | caption="2p32, resolution 3.200Å" /> | ||
'''Crystal structure of the C-terminal 10 kDa subdomain from C. elegans Hsp70'''<br /> | '''Crystal structure of the C-terminal 10 kDa subdomain from C. elegans Hsp70'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2P32 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2P32 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P32 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: three-helix bundle]] | [[Category: three-helix bundle]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:19:51 2008'' |
Revision as of 13:19, 23 January 2008
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Crystal structure of the C-terminal 10 kDa subdomain from C. elegans Hsp70
Overview
Hsp70 chaperones are composed of two domains; the 40kDa N-terminal, nucleotide-binding domain (NDB) and the 30kDa C-terminal substrate-binding, domain (SBD). Structures of the SBD from Escherichia coli homologues DnaK, and HscA show it can be further divided into an 18kDa beta-sandwich, subdomain, which forms the hydrophobic binding pocket, and a 10kDa, C-terminal three-helix bundle that forms a lid over the binding pocket., Across prokaryotes and eukaryotes, the NBD and beta-sandwich subdomain are, well conserved in both sequence and structure. The C-terminal subdomain, is, however, more evolutionary variable and the only eukaryotic structure, from rat Hsc70 revealed a diverged helix-loop-helix fold. We have solved, the crystal structure of the C-terminal 10kDa subdomain from, Caenorhabditis elegans Hsp70 which forms a helical-bundle similar to the, prokaryotic homologues. This provides the first confirmation of the, structural conservation of this subdomain in eukaryotes. Comparison with, the rat structure reveals a domain-swap dimerisation mechanism; however, the C. elegans subdomain exists exclusively as a monomer in solution in, agreement with the hypothesis that regions out with the C-terminal, subdomain are necessary for Hsp70 self-association.
About this Structure
2P32 is a Single protein structure of sequence from Caenorhabditis elegans with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of the C-terminal three-helix bundle subdomain of C. elegans Hsp70., Worrall LJ, Walkinshaw MD, Biochem Biophys Res Commun. 2007 Mar 28;. PMID:17407764
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