2hh2
From Proteopedia
(New page: 200px<br /><applet load="2hh2" size="450" color="white" frame="true" align="right" spinBox="true" caption="2hh2" /> '''Solution structure of the fourth KH domain o...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:2hh2.jpg|left|200px]]<br /><applet load="2hh2" size=" | + | [[Image:2hh2.jpg|left|200px]]<br /><applet load="2hh2" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2hh2" /> | caption="2hh2" /> | ||
'''Solution structure of the fourth KH domain of KSRP'''<br /> | '''Solution structure of the fourth KH domain of KSRP'''<br /> | ||
| Line 7: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2HH2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 2HH2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HH2 OCA]. |
==Reference== | ==Reference== | ||
| Line 16: | Line 16: | ||
[[Category: kh-rna binding domain]] | [[Category: kh-rna binding domain]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:20:34 2008'' |
Revision as of 13:20, 23 January 2008
|
Solution structure of the fourth KH domain of KSRP
Overview
The AU-rich element (ARE) RNA-binding protein KSRP (K-homology splicing, regulator protein) contains four KH domains and promotes the degradation, of specific mRNAs that encode proteins with functions in cellular, proliferation and inflammatory response. The fourth KH domain (KH4) is, essential for mRNA recognition and decay but requires the third KH domain, (KH3) for its function. We show that KH3 and KH4 behave as independent, binding modules and can interact with different regions of the AU-rich RNA, targets of KSRP. This provides KSRP with the structural flexibility needed, to recognize a set of different targets in the context of their 3'UTR, structural settings. Surprisingly, we find that KH4 binds to its target, AREs with lower affinity than KH3 and that KSRP's mRNA binding, and mRNA, degradation activities are closely associated with a conserved structural, element of KH4.
About this Structure
2HH2 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
The structure of the C-terminal KH domains of KSRP reveals a noncanonical motif important for mRNA degradation., Garcia-Mayoral MF, Hollingworth D, Masino L, Diaz-Moreno I, Kelly G, Gherzi R, Chou CF, Chen CY, Ramos A, Structure. 2007 Apr;15(4):485-98. PMID:17437720
Page seeded by OCA on Wed Jan 23 15:20:34 2008
