2ywp
From Proteopedia
(New page: 200px<br /> <applet load="2ywp" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ywp, resolution 2.90Å" /> '''Crystal Structure o...) |
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caption="2ywp, resolution 2.90Å" /> | caption="2ywp, resolution 2.90Å" /> | ||
'''Crystal Structure of CHK1 with a Urea Inhibitor'''<br /> | '''Crystal Structure of CHK1 with a Urea Inhibitor'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2YWP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with A42 as [http://en.wikipedia.org/wiki/ligand ligand]. This structure superseeds the now removed PDB entry 2FGA. Active as [http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] Full crystallographic information is available from [http:// | + | 2YWP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=A42:'>A42</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. This structure superseeds the now removed PDB entry 2FGA. Active as [http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YWP OCA]. |
==Reference== | ==Reference== | ||
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[[Category: protein-inhibitor complex]] | [[Category: protein-inhibitor complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:23:09 2008'' |
Revision as of 13:23, 23 January 2008
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Crystal Structure of CHK1 with a Urea Inhibitor
Overview
Based on the X-ray crystallography of our lead compound, 1-(5-chloro-2,4-dimethoxyphenyl)-3-(5-cyanopyrazin-2-yl)urea in the, checkpoint kinase 1 (Chk1) enzyme, we modified R4, and to a lesser extent, R2, and R5 of the phenyl ring, and made a variety of, N-aryl-N'-pyrazinylurea Chk1 inhibitors. Enzymatic activity less than 20, nM was observed in 15 of 41 compounds. Compound 8i provided the best, overall results in the cellular assays as it abrogated doxorubicin-induced, cell cycle arrest (IC50=1.7 microM) and enhanced doxorubicin cytotoxicity, (IC50=0.44 microM) while displaying no single agent activity.
About this Structure
2YWP is a Single protein structure of sequence from Homo sapiens with as ligand. This structure superseeds the now removed PDB entry 2FGA. Active as Non-specific serine/threonine protein kinase, with EC number 2.7.11.1 Full crystallographic information is available from OCA.
Reference
Synthesis and biological evaluation of 1-(2,4,5-trisubstituted phenyl)-3-(5-cyanopyrazin-2-yl)ureas as potent Chk1 kinase inhibitors., Li G, Hasvold LA, Tao ZF, Wang GT, Gwaltney SL 2nd, Patel J, Kovar P, Credo RB, Chen Z, Zhang H, Park C, Sham HL, Sowin T, Rosenberg SH, Lin NH, Bioorg Med Chem Lett. 2006 Apr 15;16(8):2293-8. Epub 2006 Jan 30. PMID:16446090
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