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Semisynthetic Ribonuclease A

2-D Semisynthetic RNase A

Introduction

The synthesis of a fully active semisynthetic RNase A supports the hypothesis that the amino acid sequence of a protein solely dictates the formation of an active enzyme and demonstrates that an enzyme with the catalytic activity and specificity of a naturally produced enzyme can be made in laboratory. Semisynthetic RNase A illustrates that functional enzymes can be produced from merely the individual constituent amino acid residues. Polypeptide synthesis can provide new routes to the study of enzymes through the selective modification of natural proteins to assay individual roles of amino acids in folding and catalysis.

Function

The structure to function relationship is clearly exhibited by semisynthetic RNase A. In the RNase A protein, the removal of six C terminal residues, leaving , completely halts enzymatic activity (Martin, 1987). However, a complex of RNase 1-118 with a synthetic polypeptide comprising the restores enzymatic activity to RNase A. Upon the addition of the synthetic chain, the semisynthetic enzyme adopts a structure that closely resembles that of natural RNase (Martin, 1987). The restoration of the structure reconstitutes the enzymatic activity of RNase to 98% (Martin, 1987).

Synthetic Method

The RNase 1-118 was prepared by successive digestion of RNase A pepsin and

carboxypeptidase A (Doscher, 1983). The synthetic component, RNase 111-124, 

was prepared by the use of solid-phase peptide synthetic mothods, in which

the peptide chain was assembled in the stepwise mannar while it was attached 

at one end to a solid support. The peptide chain was extented by repetitive steps of deprotection, neutralization and coupling until the desired sequence was obtained (Lin, 1970). It was important that the synthesis proceeds rapidly and in high yields to prevent side reactions or by-products.

Related Web-links

 1. Introduction to Ribonuclease A by Raines: 

http://www.uta.edu/faculty/sawasthi/Enzymology-4351-5324/Class%20Syllabus%20Enzymology/ribonucleaseA.pdf

 2. Solid Phase Synthesis by Merrifield (Nobel Prize Winner):

http://nobelprize.org/nobel_prizes/chemistry/laureates/1984/merrifield-lecture.pdf

 3. Chemical Synthesis of Proteins:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2845543/?tool=pmcentrez
 4. Refined Crystal Structure: http://www.ncbi.nlm.nih.gov/pubmed/3680234

References

Martin, Philip D., Marilynn S. Doscher, and Brian F. P. Edwards. "The Redefined

Crystal Structure of a Fully Active Semisynthetic Ribonuclease at 1.8-A Resolution." The Journal of Biological Chemistry 262.33 (1987): 15930-5938.

Marilynn S. Doscher, Philip D. Martin and Brian F.P. Edwards, "Characerization

of the Histidine Proton Nuclear Magnetic Resonance of a Semisynthetic Ribonuclease." Biochemistry, 1983,22,4125-4131.

Lin, M. C. (1970) Journal of Biological Chemistry, 245, 6726-6731.

David J. Boerema, Valentina. A. T., Stephen B. H. Kent, "Total Synthesis by

Modern chemical Ligation Methods and High Resolution (1.1-A) X-ray structure of Ribonuclease A. Biopolymers. 2008;90(3):278-86. Link title