2nye
From Proteopedia
(New page: 200px<br /><applet load="2nye" size="450" color="white" frame="true" align="right" spinBox="true" caption="2nye, resolution 2.5Å" /> '''Crystal structure of ...) |
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'''Crystal structure of the Bateman2 domain of yeast Snf4'''<br /> | '''Crystal structure of the Bateman2 domain of yeast Snf4'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2NYE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http:// | + | 2NYE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NYE OCA]. |
==Reference== | ==Reference== | ||
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[[Category: snf4]] | [[Category: snf4]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:24:35 2008'' |
Revision as of 13:24, 23 January 2008
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Crystal structure of the Bateman2 domain of yeast Snf4
Overview
AMP-activated protein kinase (AMPK) is a central regulator of energy, homeostasis in mammals. AMP is believed to control the activity of AMPK by, binding to the gamma subunit of this heterotrimeric enzyme. This subunit, contains two Bateman domains, each of which is composed of a tandem pair, of cystathionine beta-synthase (CBS) motifs. No structural information is, currently available on this subunit, and the molecular basis for its, interactions with AMP is not well understood. We report here the crystal, structure at 1.9 Angstrom resolution of the Bateman2 domain of Snf4, the, gamma subunit of the yeast ortholog of AMPK. The structure revealed a, dimer of the Bateman2 domain, and this dimerization is supported by our, light-scattering, mutagenesis, and biochemical studies. There is a, prominent pocket at the center of this dimer, and most of the, disease-causing mutations are located in or near this pocket.
About this Structure
2NYE is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Structure of the Bateman2 domain of yeast Snf4: dimeric association and relevance for AMP binding., Rudolph MJ, Amodeo GA, Iram SH, Hong SP, Pirino G, Carlson M, Tong L, Structure. 2007 Jan;15(1):65-74. PMID:17223533
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