2o6d
From Proteopedia
(New page: 200px<br /><applet load="2o6d" size="450" color="white" frame="true" align="right" spinBox="true" caption="2o6d, resolution 1.860Å" /> '''Structure of native...) |
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| - | [[Image:2o6d.gif|left|200px]]<br /><applet load="2o6d" size=" | + | [[Image:2o6d.gif|left|200px]]<br /><applet load="2o6d" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2o6d, resolution 1.860Å" /> | caption="2o6d, resolution 1.860Å" /> | ||
'''Structure of native rTp34 from Treponema pallidum'''<br /> | '''Structure of native rTp34 from Treponema pallidum'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2O6D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Treponema_pallidum Treponema pallidum] with ZN, SO4, CL and EDO as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 2O6D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Treponema_pallidum Treponema pallidum] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=EDO:'>EDO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O6D OCA]. |
==Reference== | ==Reference== | ||
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[[Category: syphilis]] | [[Category: syphilis]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:24:39 2008'' |
Revision as of 13:24, 23 January 2008
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Structure of native rTp34 from Treponema pallidum
Overview
The Tp34 (TP0971) membrane lipoprotein of Treponema pallidum, an obligate, human pathogen and the agent of syphilis, was previously reported to have, lactoferrin binding properties. Given the non-cultivatable nature of T., pallidum, a structure-to-function approach was pursued to clarify further, potential relationships between the Tp34 structural and biochemical, properties and its propensity to bind human lactoferrin. The crystal, structure of a nonacylated, recombinant form of Tp34 (rTp34), solved to a, resolution of 1.9A(,) revealed two metaloccupied binding sites within a, dimer; the identity of the ion most likely was zinc. Residues from both of, the monomers contributed to the interfacial metal-binding sites; a novel, feature was that the delta-sulfur of methionine coordinated the zinc ion., Analytical ultracentrifugation showed that, in solution, rTp34 formed a, metal-stabilized dimer and that rTp34 bound human lactoferrin with a, stoichiometry of 2:1. Isothermal titration calorimetry further revealed, that rTp34 bound human lactoferrin at high (submicromolar) affinity., Finally, membrane topology studies revealed that native Tp34 is not, located on the outer surface (outer membrane) of T. pallidum but, rather, is periplasmic. How propensity of Tp34 to bind zinc and the, iron-sequestering lactoferrin may relate overall to the biology of T., pallidum infection in humans is discussed.
About this Structure
2O6D is a Single protein structure of sequence from Treponema pallidum with , , and as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of the Tp34 (TP0971) lipoprotein of treponema pallidum: implications of its metal-bound state and affinity for human lactoferrin., Deka RK, Brautigam CA, Tomson FL, Lumpkins SB, Tomchick DR, Machius M, Norgard MV, J Biol Chem. 2007 Feb 23;282(8):5944-58. Epub 2006 Dec 27. PMID:17192261
Page seeded by OCA on Wed Jan 23 15:24:39 2008
Categories: Single protein | Treponema pallidum | Brautigam, C.A. | Deka, R.K. | Lumpkins, S.B. | Machius, M. | Norgard, M.V. | Tomchick, D.R. | CL | EDO | SO4 | ZN | Dimer | Ig-fold | Metal-ion binding | Syphilis
