2gxb
From Proteopedia
(New page: 200px<br /> <applet load="2gxb" size="450" color="white" frame="true" align="right" spinBox="true" caption="2gxb, resolution 2.25Å" /> '''Crystal Structure o...) |
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| - | [[Image:2gxb.gif|left|200px]]<br /> | + | [[Image:2gxb.gif|left|200px]]<br /><applet load="2gxb" size="350" color="white" frame="true" align="right" spinBox="true" |
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caption="2gxb, resolution 2.25Å" /> | caption="2gxb, resolution 2.25Å" /> | ||
'''Crystal Structure of The Za Domain bound to Z-RNA'''<br /> | '''Crystal Structure of The Za Domain bound to Z-RNA'''<br /> | ||
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==Overview== | ==Overview== | ||
The A form RNA double helix can be transformed to a left-handed helix, called Z-RNA. Currently, little is known about the detailed structural, features of Z-RNA or its involvement in cellular processes. The discovery, that certain interferon-response proteins have domains that can stabilize, Z-RNA as well as Z-DNA opens the way for the study of Z-RNA. Here, we, present the 2.25 A crystal structure of the Zalpha domain of the, RNA-editing enzyme ADAR1 (double-stranded RNA adenosine deaminase), complexed to a dUr(CG)(3) duplex RNA. The Z-RNA helix is associated with a, unique solvent pattern that distinguishes it from the otherwise similar, conformation of Z-DNA. Based on the structure, we propose a model, suggesting how differences in solvation lead to two types of Z-RNA, structures. The interaction of Zalpha with Z-RNA demonstrates how the, interferon-induced isoform of ADAR1 could be targeted toward selected, dsRNAs containing purine-pyrimidine repeats, possibly of viral origin. | The A form RNA double helix can be transformed to a left-handed helix, called Z-RNA. Currently, little is known about the detailed structural, features of Z-RNA or its involvement in cellular processes. The discovery, that certain interferon-response proteins have domains that can stabilize, Z-RNA as well as Z-DNA opens the way for the study of Z-RNA. Here, we, present the 2.25 A crystal structure of the Zalpha domain of the, RNA-editing enzyme ADAR1 (double-stranded RNA adenosine deaminase), complexed to a dUr(CG)(3) duplex RNA. The Z-RNA helix is associated with a, unique solvent pattern that distinguishes it from the otherwise similar, conformation of Z-DNA. Based on the structure, we propose a model, suggesting how differences in solvation lead to two types of Z-RNA, structures. The interaction of Zalpha with Z-RNA demonstrates how the, interferon-induced isoform of ADAR1 could be targeted toward selected, dsRNAs containing purine-pyrimidine repeats, possibly of viral origin. | ||
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| - | ==Disease== | ||
| - | Known diseases associated with this structure: Dyschromatosis symmetrica hereditaria OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=601059 601059]] | ||
==About this Structure== | ==About this Structure== | ||
| - | 2GXB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with NA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2GXB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=NA:'>NA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GXB OCA]. |
==Reference== | ==Reference== | ||
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[[Category: za]] | [[Category: za]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:26:52 2008'' |
Revision as of 13:26, 23 January 2008
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Crystal Structure of The Za Domain bound to Z-RNA
Overview
The A form RNA double helix can be transformed to a left-handed helix, called Z-RNA. Currently, little is known about the detailed structural, features of Z-RNA or its involvement in cellular processes. The discovery, that certain interferon-response proteins have domains that can stabilize, Z-RNA as well as Z-DNA opens the way for the study of Z-RNA. Here, we, present the 2.25 A crystal structure of the Zalpha domain of the, RNA-editing enzyme ADAR1 (double-stranded RNA adenosine deaminase), complexed to a dUr(CG)(3) duplex RNA. The Z-RNA helix is associated with a, unique solvent pattern that distinguishes it from the otherwise similar, conformation of Z-DNA. Based on the structure, we propose a model, suggesting how differences in solvation lead to two types of Z-RNA, structures. The interaction of Zalpha with Z-RNA demonstrates how the, interferon-induced isoform of ADAR1 could be targeted toward selected, dsRNAs containing purine-pyrimidine repeats, possibly of viral origin.
About this Structure
2GXB is a Single protein structure of sequence from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
Reference
A Left-Handed RNA Double Helix Bound by the Zalpha Domain of the RNA-Editing Enzyme ADAR1., Placido D, Brown BA 2nd, Lowenhaupt K, Rich A, Athanasiadis A, Structure. 2007 Apr;15(4):395-404. PMID:17437712
Page seeded by OCA on Wed Jan 23 15:26:52 2008
Categories: Homo sapiens | Single protein | Athanasiadis, A. | Placido, D. | Rich, A. | NA | Adar1 | Protein-rna complex | Rna editing | Z-rna | Za
