2pnf
From Proteopedia
(New page: 200px<br /><applet load="2pnf" size="450" color="white" frame="true" align="right" spinBox="true" caption="2pnf, resolution 1.80Å" /> '''Structure of Aquifex...) |
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| - | [[Image:2pnf.jpg|left|200px]]<br /><applet load="2pnf" size=" | + | [[Image:2pnf.jpg|left|200px]]<br /><applet load="2pnf" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2pnf, resolution 1.80Å" /> | caption="2pnf, resolution 1.80Å" /> | ||
'''Structure of Aquifex Aeolicus FabG 3-oxoacyl-(acyl-carrier protein) reductase'''<br /> | '''Structure of Aquifex Aeolicus FabG 3-oxoacyl-(acyl-carrier protein) reductase'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2PNF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus] with 1PE and MES as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/3-oxoacyl-[acyl-carrier-protein]_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.100 1.1.1.100] Full crystallographic information is available from [http:// | + | 2PNF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus] with <scene name='pdbligand=1PE:'>1PE</scene> and <scene name='pdbligand=MES:'>MES</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/3-oxoacyl-[acyl-carrier-protein]_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.100 1.1.1.100] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PNF OCA]. |
==Reference== | ==Reference== | ||
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[[Category: short chain oxidoreductase]] | [[Category: short chain oxidoreductase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:29:33 2008'' |
Revision as of 13:29, 23 January 2008
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Structure of Aquifex Aeolicus FabG 3-oxoacyl-(acyl-carrier protein) reductase
Overview
The gene product of fabG from Aquifex aeolicus has been heterologously, expressed in Escherichia coli. Purification of the protein took place, using anion-exchange and size-exclusion chromatography and the protein was, then crystallized. Diffraction data were collected to a maximum resolution, of 1.8 A and the initial phases were determined by molecular replacement., The A. aeolicus FabG protein is a putative beta-ketoacyl-acyl carrier, protein reductase. Structure-function studies of this protein are being, performed as part of a larger project investigating naturally occurring, deviations from highly conserved residues within the short-chain, oxidoreductase (SCOR) family.
About this Structure
2PNF is a Single protein structure of sequence from Aquifex aeolicus with and as ligands. Active as [acyl-carrier-protein_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number 1.1.1.100 Full crystallographic information is available from OCA.
Reference
Crystallization and X-ray diffraction analysis of the beta-ketoacyl-acyl carrier protein reductase FabG from Aquifex aeolicus VF5., Mao Q, Duax WL, Umland TC, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Feb 1;63(Pt, 2):106-9. Epub 2007 Jan 17. PMID:17277451 [[Category: 3-oxoacyl-[acyl-carrier-protein] reductase]]
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