2flg
From Proteopedia
(New page: 200px<br /><applet load="2flg" size="450" color="white" frame="true" align="right" spinBox="true" caption="2flg" /> '''Solution structure of an EGF-LIKE domain fro...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:2flg.gif|left|200px]]<br /><applet load="2flg" size=" | + | [[Image:2flg.gif|left|200px]]<br /><applet load="2flg" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2flg" /> | caption="2flg" /> | ||
'''Solution structure of an EGF-LIKE domain from the Plasmodium falciparum merozoite surface protein 1'''<br /> | '''Solution structure of an EGF-LIKE domain from the Plasmodium falciparum merozoite surface protein 1'''<br /> | ||
| Line 7: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2FLG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with NH2 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2FLG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=NH2:'>NH2</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FLG OCA]. |
==Reference== | ==Reference== | ||
| Line 24: | Line 24: | ||
[[Category: surface protein]] | [[Category: surface protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:30:04 2008'' |
Revision as of 13:30, 23 January 2008
|
Solution structure of an EGF-LIKE domain from the Plasmodium falciparum merozoite surface protein 1
Overview
The Plasmodium falciparum merozoite surface protein-1 19 kDa fragment, (MSP-1(19)) comprises two closely packed EGF-like domains (EGF=epidermal, growth factor), each stabilized by three disulfide bonds. The native, conformation of this protein is important for eliciting P. falciparum, growth inhibitory antibodies. Here we show that the N-terminal EGF domain, alone can be chemically synthesized and efficiently refolded to a, native-like state, as shown by its solution structure as determined by NMR, spectroscopy. In order to study its immunogenicity, the domain was coupled, through its N terminus to a phospholipid and incorporated into, reconstituted influenza virus-like particles (virosomes). When used to, immunize mice, the peptide-loaded virosomes elicited potent humoral immune, responses that were shown by Western blots and immunofluorescence assays, to cross-react with native MSP-1 on the surfaces of P. falciparum blood, stage parasites. This opens the way for a medicinal chemistry-oriented, approach to the study and optimization of the antigenicity of the protein, as a potential malaria vaccine candidate, whilst exploiting the, immunopotentiating properties of influenza virosomes as a delivery, vehicle.
About this Structure
2FLG is a Single protein structure of sequence from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
Synthesis, solution structure and immune recognition of an epidermal growth factor-like domain from Plasmodium falciparum merozoite surface protein-1., James S, Moehle K, Renard A, Mueller MS, Vogel D, Zurbriggen R, Pluschke G, Robinson JA, Chembiochem. 2006 Dec;7(12):1943-50. PMID:17068840
Page seeded by OCA on Wed Jan 23 15:30:04 2008
