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===INTRODUCTION===
===INTRODUCTION===
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Transcription is the process of mRNA synthesis by RNA polymerase, an enzyme that uses a strand of DNA as a template for ribonucloetide addition. During this process, RNA polymerase encounters several regulatory proteins, called transcription factors, that effect transcription in various ways. One of the major regulatory activities of transcription regulators is terminating the process at specific sites in the DNA. In ''E. coli'', transcription is terminated through rho dependent and independent mechanisms. Rho dependent transcription requires a helicase protein called rho factor. Rho factor has helicase activity as it functions by moving on the new mRNA strand toward the RNA polymerase, DNA and RNA complex whose dissociation results in the termination of transcription. Rho uses energy derived from its ATPase mechanism (hydrolysis of ATP into ADP and pyrophosphate) to drive its movement along the newly formed RNA molecule toward the elongation complex to be dissociated.
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Transcription is the process of mRNA synthesis by RNA polymerase, an enzyme that uses a strand of DNA as a template for ribonucloetide addition. During this process, RNA polymerase encounters several regulatory proteins, called transcription factors, that effect transcription in various ways. One of the major regulatory activities of transcription regulators is terminating the process at specific sites in the DNA. In ''E. coli'', transcription is terminated through rho dependent and independent mechanisms. Rho dependent transcription requires a helicase protein called rho factor. Rho factor belongs in the helicase class of enzymes because it is a motor protein that functions through directional movement on the new mRNA strand toward the RNA polymerase, DNA and RNA complex (elongation complex) whose dissociation results in the termination of transcription. It uses energy derived from its ATPase mechanism (hydrolysis of ATP into ADP and pyrophosphate) to drive its movement along the newly formed RNA molecule toward the elongation complex to be dissociated.
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Revision as of 05:00, 23 April 2011

Rho Termination factor with its six identical subunits in various colors
Rho Termination factor with its six identical subunits in various colors

Contents

RHO TERMINATION FACTOR


INTRODUCTION

Transcription is the process of mRNA synthesis by RNA polymerase, an enzyme that uses a strand of DNA as a template for ribonucloetide addition. During this process, RNA polymerase encounters several regulatory proteins, called transcription factors, that effect transcription in various ways. One of the major regulatory activities of transcription regulators is terminating the process at specific sites in the DNA. In E. coli, transcription is terminated through rho dependent and independent mechanisms. Rho dependent transcription requires a helicase protein called rho factor. Rho factor belongs in the helicase class of enzymes because it is a motor protein that functions through directional movement on the new mRNA strand toward the RNA polymerase, DNA and RNA complex (elongation complex) whose dissociation results in the termination of transcription. It uses energy derived from its ATPase mechanism (hydrolysis of ATP into ADP and pyrophosphate) to drive its movement along the newly formed RNA molecule toward the elongation complex to be dissociated.


HISTORY


STRUCTURE

Rho factor in its hexameric form with its six identical subunits forming a helicase and an RNA molecule right through the center

Drag the structure with the mouse to rotate
Rho factor is a hexamer of six identical subunits that interact to form a closed, dynamic structure called a

INHIBITION


Proteopedia Page Contributors and Editors (what is this?)

Momodou L. Jammeh

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