2hyr
From Proteopedia
(New page: 200px<br /> <applet load="2hyr" size="450" color="white" frame="true" align="right" spinBox="true" caption="2hyr, resolution 1.51Å" /> '''Crystal structure o...) |
|||
Line 1: | Line 1: | ||
- | [[Image:2hyr.gif|left|200px]]<br /> | + | [[Image:2hyr.gif|left|200px]]<br /><applet load="2hyr" size="350" color="white" frame="true" align="right" spinBox="true" |
- | <applet load="2hyr" size=" | + | |
caption="2hyr, resolution 1.51Å" /> | caption="2hyr, resolution 1.51Å" /> | ||
'''Crystal structure of a complex of griffithsin with maltose'''<br /> | '''Crystal structure of a complex of griffithsin with maltose'''<br /> | ||
Line 8: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
- | 2HYR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Griffithsia Griffithsia] with SO4, MG and EDO as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 2HYR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Griffithsia Griffithsia] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=EDO:'>EDO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HYR OCA]. |
==Reference== | ==Reference== | ||
Line 26: | Line 25: | ||
[[Category: sars]] | [[Category: sars]] | ||
- | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:35:05 2008'' |
Revision as of 13:35, 23 January 2008
|
Crystal structure of a complex of griffithsin with maltose
Overview
The mode of binding of oligosaccharides to griffithsin, an antiviral, lectin from the red alga Griffithsia sp., was investigated by a, combination of X-ray crystallography, isothermal titration calorimetry, and molecular modeling. The structures of complexes of griffithsin with, 1-->6alpha-mannobiose and with maltose were solved and refined at the, resolution of 2.0 and 1.5 A, respectively. The thermodynamic parameters of, binding of 1-->6alpha-mannobiose, maltose, and mannose to griffithsin were, determined. Binding profiles of 1-->6alpha-mannobiose and mannose were, similar with Kd values of 83.3 microM and 102 microM, respectively. The, binding of maltose to griffithsin was significantly weaker, with a, fourfold lower affinity (Kd = 394 microM). In all cases the binding at 30, degrees C was entropically rather than enthalpically driven. On the basis, of the experimental crystal structures, as well as on previously, determined structures of complexes with monosaccharides, it was possible, to create a model of a tridentate complex of griffithsin with Man9GlcNAc2, a high mannose oligosaccharide commonly found on the surface of viral, glycoproteins. All shorter oligomannoses could be modeled only as, bidentate or monodentate complexes with griffithsin. The ability to, mediate tight multivalent and multisite interactions with high-mannose, oligosaccharides helps to explain the potent antiviral activity of, griffithsin.
About this Structure
2HYR is a Single protein structure of sequence from Griffithsia with , and as ligands. Full crystallographic information is available from OCA.
Reference
Crystallographic, thermodynamic, and molecular modeling studies of the mode of binding of oligosaccharides to the potent antiviral protein griffithsin., Ziolkowska NE, Shenoy SR, O'Keefe BR, McMahon JB, Palmer KE, Dwek RA, Wormald MR, Wlodawer A, Proteins. 2007 May 15;67(3):661-70. PMID:17340634
Page seeded by OCA on Wed Jan 23 15:35:05 2008
Categories: Griffithsia | Single protein | Wlodawer, A. | Ziolkowska, N.E. | EDO | MG | SO4 | Domain swapping | Griffithsin | Hiv | Lectins | Mannose binding | Sars