2jmc
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(New page: 200px<br /><applet load="2jmc" size="350" color="white" frame="true" align="right" spinBox="true" caption="2jmc" /> '''Chimer between Spc-SH3 and P41'''<br /> ==O...)
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Revision as of 13:35, 23 January 2008
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Chimer between Spc-SH3 and P41
Overview
Here we present the high-resolution NMR structure of a chimera (SPCp41), between alpha-spectrin SH3 domain and the decapeptide p41. The tertiary, structure mimics perfectly the interactions typically found in SH3-peptide, complexes and is remarkably similar to that of the complex between the, separate Spc-SH3 domain and ligand p41. Relaxation data confirm the tight, binding between the ligand and SH3 part of the chimera. This chimera will, serve as a tool for a deeper understanding of the relationship between, structure and thermodynamics of binding using a combination of NMR, stability and site-directed mutagenesis studies, which can lead to an, effective strategy for ligand design.
About this Structure
2JMC is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.
Reference
The high-resolution NMR structure of a single-chain chimeric protein mimicking a SH3-peptide complex., Candel AM, Conejero-Lara F, Martinez JC, van Nuland NA, Bruix M, FEBS Lett. 2007 Feb 20;581(4):687-92. Epub 2007 Jan 22. PMID:17275816
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